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Control transcripcional de la vía específica masculina en la determinación del sexo dependiente de la temperatura

Yin-Zi Ye¹, Jiameng Li², Weiming Li³

¹State Key Laboratory of Wetland Conservation and Restoration, National Observations and Research Station for Wetland Ecosystems of the Yangtze Estuary, Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, and Institute of Eco-Chongming, School of Life Sciences, Fudan University, Shanghai 200438, China; College of Wildlife and Protected Area, Northeast Forestry University, Harbin 150040, China; Heilongjiang Key Laboratory of Complex Traits and Protein Machines in Organisms, Harbin 150040, China.

Science bulletin

|August 29, 2025

Resumen

Este estudio descubre la vía molecular que la temperatura utiliza para desencadenar el desarrollo masculino en las tortugas. Las temperaturas frías activan una nueva cascada de señalización, lo que lleva a la determinación del sexo masculino en especies de determinación del sexo dependientes de la temperatura.

Área de la Ciencia:

Biología de la reproducción
Biología del desarrollo
Endocrinología

Sus antecedentes:

La determinación del sexo dependiente de la temperatura (TSD) es crucial en los reptiles, pero los mecanismos moleculares subyacentes siguen siendo en gran medida desconocidos.
Investigaciones anteriores identificaron a las células esteroideogénicas termo-sensibles (TSSC) como actores clave en el desencadenamiento del desarrollo masculino en las tortugas.

Objetivo del estudio:

Elucidar los mecanismos reguladores de la transcripción que vinculan la sensibilidad al frío de la TSSC con la especificación de las células pre-Sertoli.
Descubrir la nueva vía específica masculina iniciada por la temperatura para la determinación del sexo masculino en las especies de TSD.

Principales métodos:

Se ha investigado la síntesis de aldosterona inducida por Ca2+ en las TSSC.
Se analizó la modulación transcripcional impulsada por la aldosterona en las células pre-suportantes.
Se examinó el papel de la expresión génica NR3C1 y DMRT1 ligada a la aldosterona.

Principales resultados:

Se descubrió una nueva vía que involucra la síntesis de aldosterona inducida por Ca2+ en las TSSC.
Se ha demostrado la modulación de la transcripción impulsada por la aldosterona en las células pre-suportantes.
Se identificó el NR3C1 ligado a la aldosterona como un regulador maestro que activa la expresión del gen DMRT1 para la especificación previa a Sertoli.

Conclusiones:

Se aclaró el esquema regulatorio para la vía de determinación masculina en tortugas TSD.
Este estudio revela una nueva vía de determinación del sexo masculino iniciada por la temperatura.

Palabras clave:

Aldosterona y sus derivados Vía de determinación de los machos Secuenciación de ARN de una sola célula Determinación del sexo dependiente de la temperatura Células esteroideas térmicamente sensibles Trachemys scripta elegans, también conocido como

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