Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Epigenetic Regulation01:37

Epigenetic Regulation

3.1K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
3.1K
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

1.9K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
1.9K
Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

1.9K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
1.9K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

994
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
994
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.3K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.3K
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

3.1K
3.1K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Synthesis of bisindole scaffolds by palladium-catalyzed allylic alkylation of <i>N</i>-aminoindole with <i>ortho</i>-iodoaryl allenes.

Organic & biomolecular chemistry·2026
Same author

FTO Controls Endometrial Receptivity and Embryo Implantation through Regulating m<sup>6</sup>A and H3K27me3.

Communications biology·2026
Same author

Ythdf2/Setd1b regulatory axis is essential for cerebellar development through regulating epigenetic reprogramming.

Molecular psychiatry·2026
Same author

METTL3 Affects Endometrial Receptivity Through Modulation of P62-Mediated Autophagic Flux.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2025
Same author

RNA-m5C regulatory atlas of human fetal tissues uncover the activities of Nsun2/Jarid2/Alyref axis.

Journal of advanced research·2025
Same author

An overview of RecQ helicases and related diseases.

Aging·2025

Video Experimental Relacionado

Updated: Sep 9, 2025

Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry
08:45

Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry

Published on: April 21, 2022

2.5K

Una modificación es esencial para el desarrollo cerebeloso a través de la regulación de la reprogramación epigenética

Jing Jiang1, Ming Zhang1, Wenjuan Xia1

  • 1State Key Laboratory of Reproductive Medicine and Offspring Health (Suzhou Centre), Suzhou Municipal Hospital, Gusu School, Suzhou Affiliated Hospital of Nanjing Medical University, Nanjing Medical University, Suzhou, 215002, China.

Journal of biomedical science
|August 29, 2025
PubMed
Resumen

La pérdida del gen Fto en ratones causó ataxia cerebelosa debido a una regulación epitranscriptómica alterada. Este estudio revela que el

Palabras clave:
FtoNo lo sé.Desarrollo del cerebeloH4K16ac y sus derivadosPalabras claveM6A modificación

Más Videos Relacionados

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
08:56

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

Published on: December 5, 2016

11.0K
Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis
08:50

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

Published on: May 14, 2020

6.8K

Videos de Experimentos Relacionados

Last Updated: Sep 9, 2025

Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry
08:45

Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry

Published on: April 21, 2022

2.5K
A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
08:56

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

Published on: December 5, 2016

11.0K
Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis
08:50

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

Published on: May 14, 2020

6.8K

Área de la Ciencia:

  • La neurociencia
  • La epigenética
  • Biología molecular

Sus antecedentes:

  • La regulación epitranscriptómica, particularmente la metilación, es crucial para el desarrollo y la función cerebelosa.
  • El papel específico de la ARN desmetilasa Fto en el cerebelo sigue sin estar claro.

Objetivo del estudio:

  • Investigar la función de Fto en el desarrollo y la función cerebelosa utilizando un modelo de ratón knockout.
  • Aclarar los mecanismos moleculares subyacentes al papel de Fto en el desarrollo cerebeloso.

Principales métodos:

  • Se generaron ratones Fto knockout (Fto KO) para el análisis fenotípico.
  • Evaluación de la función cerebelosa mediante pruebas de comportamiento y coloración Nissl.
  • Utilizó técnicas moleculares que incluyen inmunofluorescencia, m6A-RIP-seq, ATAC-seq, CUT&Tag-seq y Co-IP para analizar la expresión génica, los niveles de m6A y la accesibilidad de la cromatina.

Principales resultados:

  • Los ratones FtoKO mostraron ataxia cerebelosa con temblores y una marcha anormal.
  • La expresión reducida de FTO condujo a una expresión alterada del desarrollo neuronal y de los genes de auto-renovación.
  • Mecanísticamente, la pérdida de Fto dio lugar a una regulación al alza de Kat8, aumentó los niveles de m6A y alteró la modificación de H4K16ac, lo que afectó la accesibilidad de la cromatina.

Conclusiones:

  • Fto juega un papel crítico en el desarrollo cerebeloso.
  • La deficiencia de Fto interrumpe la función cerebelosa a través de la regulación dependiente de A de Kat8 y la accesibilidad a la cromatina.
  • Estos hallazgos resaltan la importancia de la regulación epitranscriptómica en los procesos de desarrollo neurológico.