Video Experimental Relacionado
Updated: Sep 9, 2025
09:40
In Vivo Detection and Analysis of Rb Protein SUMOylation in Human Cells
Published on: November 2, 2017
7.4K
La ubiquitinación vinculada a K29 de los reguladores de la transcripción controla la proliferación celular en la
Qiushuang Zhang1, Xucong Teng2,1,3, Yicong Dai2,1
1New Cornerstone Science Laboratory, Department of Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, Beijing, 100084, China.
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
|August 30, 2025
Resumen
Las cadenas de ubiquitina no canónicas ligadas a K29 regulan la transcripción génica durante la respuesta de la proteína desplegada (UPR). La ubiquitinación K29 upregulada de la cohesión interrumpe la iniciación de la transcripción, bajando la regulación de los genes de proliferación celular.
Área de la Ciencia:
- Biología celular
- Biología molecular
- La genética
Sus antecedentes:
- Las cadenas de ubiquitina tienen diversas funciones, pero las cadenas vinculadas a K29 son poco conocidas.
- La respuesta proteica desplegada (UPR) gestiona el estrés del retículo endoplasmático a través de una regulación precisa.
- El papel de la ubiquitina más allá de la degradación de proteínas en la UPR es un área activa de investigación.
Objetivo del estudio:
- Investigar las funciones no canónicas de las cadenas de ubiquitina ligadas a K29.
- Explorar el papel de las cadenas de ubiquitina vinculadas a K29 en la regulación de la transcripción durante la RPU.
- Elucidar los mecanismos reguladores de la ubiquitinación de la cohesión en las respuestas al estrés celular.
Principales métodos:
- Inducción de la respuesta proteica desplegada (UPR).
- Análisis de la ubiquitinación ligada a K29 de las proteínas del complejo de la cohesina (SMC1A y SMC3).
- Evaluación de la regulación transcripcional de los genes relacionados con la proliferación celular (SERTAD1, NUDT16L1).
Principales resultados:
- La ubiquitinación ligada a K29 de SMC1A y SMC3 aumenta con la inducción de la UPR.
- La ubiquitinación de cohesión ligada a K29 regula la transcripción de los genes de proliferación celular.
- El aumento de la ubiquitinación de la cohesión ligada a K29 interrumpe la formación del complejo de iniciación de la transcripción.
Conclusiones:
- Las cadenas de ubiquitina ligadas a K29 juegan un papel importante en la regulación de la transcripción durante la UPR.
- La ubiquitinación de la cohesina es un mecanismo clave que controla la expresión génica en respuesta al estrés ER.
- Los hallazgos revelan una nueva vía reguladora que afecta la proliferación celular bajo condiciones de estrés.
Videos de Conceptos Relacionados
01:31Regulation of the Unfolded Protein Response
2.6K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.6K
01:37The Unfolded Protein Response
5.1K
The ER is the hub of protein synthesis in a cell. It has robust systems to quality control protein folding and also for degradation of terminally misfolded proteins. Under normal conditions, a small proportion of misfolded proteins that cannot be salvaged need to be transported to the cytoplasm by the ER-associated degradation or ERAD pathways. However, if the ERAD cannot handle the misfolded proteins, the cell activates the unfolded protein response or UPR to adjust the protein folding...
5.1K
02:04Covalently Linked Protein Regulators
7.1K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
These groups modify specific amino acids in a protein....
7.1K
01:23Regulation of Expression at Multiple Steps
994
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
994
01:22PI3K/mTOR/AKT Signaling Pathway
3.9K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
3.9K
01:23Negative Regulator Molecules
35.9K
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
35.9K