Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Translational Regulation01:29

Translational Regulation

91
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
91
Riboswitches01:56

Riboswitches

8.5K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.5K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

994
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
994
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

23.2K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
23.2K
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

7.2K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
7.2K
Transcriptional Regulation: Riboswitches01:23

Transcriptional Regulation: Riboswitches

113
Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
113

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Snord67 promotes breast cancer metastasis by guiding U6 modification and modulating the splicing landscape.

Nature communications·2025
Same author

Alternative splicing of the Snap23 microexon is regulated by MBNL, QKI, and RBFOX2 in a tissue-specific manner and is altered in striated muscle diseases.

RNA biology·2025
Same author

Splicing regulation through biomolecular condensates and membraneless organelles.

Nature reviews. Molecular cell biology·2024
Same author

RBFOX2 regulated EYA3 isoforms partner with SIX4 or ZBTB1 to control transcription during myogenesis.

iScience·2023
Same author

Author Correction: It's not just a phase: function and characteristics of RNA-binding proteins in phase separation.

Nature structural & molecular biology·2022
Same author

Author Correction: Stretching muscle cells induces transcriptional and splicing transitions and changes in SR proteins.

Communications biology·2022
Same journal

Structural proteomics reveals that misfolded nascent proteins expose buried lysines for ubiquitination and rapid proteasomal degradation.

Cell reports·2026
Same journal

Physical interactions within the SIR heterochromatin complex potentiate inter-subunit communication and gene repression.

Cell reports·2026
Same journal

An IGF2BP3-dependent metabolic circuit governs macrophage recruitment and immunosuppression in glioblastoma.

Cell reports·2026
Same journal

A cold-induced GDF15-secreting adipocyte subpopulation regulates energy homeostasis through endocrine signaling.

Cell reports·2026
Same journal

RelB drives integrin-mediated stress tolerance and relapse in high-grade serous ovarian cancer.

Cell reports·2026
Same journal

Yeast β-glucan supplementation supports immunometabolic anti-tumor responses and reverses obesity-induced dysfunction via trained hematopoiesis.

Cell reports·2026
Ver todos los artículos relacionados

Video Experimental Relacionado

Updated: Sep 9, 2025

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation
12:26

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation

Published on: February 12, 2022

5.2K

La interacción bidireccional entre el procesamiento de ARN y la mecanotransducción

Gabrielle B Bais1, Jimena Giudice2

  • 1Curriculum in Cell Biology and Physiology, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA; Department of Cell Biology and Physiology, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA; Integrated Vascular Biology Training Program, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.

Cell reports
|August 30, 2025
PubMed
Resumen
Este resumen es generado por máquina.

Las células perciben las fuerzas mecánicas a través de la mecanotransducción, influyendo en la expresión génica a través del procesamiento de ARN. Esta revisión destaca cómo las señales mecánicas impactan el empalme alternativo y la poliadenilación, cruciales para las respuestas celulares.

Palabras clave:
CP: Biología celularCP: Biología molecular

Más Videos Relacionados

Nanomanipulation of Single RNA Molecules by Optical Tweezers
06:59

Nanomanipulation of Single RNA Molecules by Optical Tweezers

Published on: August 20, 2014

14.9K
Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
10:59

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events

Published on: May 13, 2019

9.8K

Videos de Experimentos Relacionados

Last Updated: Sep 9, 2025

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation
12:26

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation

Published on: February 12, 2022

5.2K
Nanomanipulation of Single RNA Molecules by Optical Tweezers
06:59

Nanomanipulation of Single RNA Molecules by Optical Tweezers

Published on: August 20, 2014

14.9K
Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
10:59

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events

Published on: May 13, 2019

9.8K

Área de la Ciencia:

  • Biología celular
  • Biología molecular
  • La genética

Sus antecedentes:

  • Las células perciben y reaccionan a los estímulos mecánicos a través de la mecanotransducción.
  • Procesamiento de ARN, incluido el empalme y la poliadenilación, afinando la expresión génica.
  • La mecanotransducción convierte estímulos mecánicos en señales bioquímicas que regulan la expresión génica.

Objetivo del estudio:

  • Revisar los hallazgos recientes sobre los vínculos entre la mecanotransducción y el procesamiento del ARN.
  • Centrarse en el impacto de las fuerzas mecánicas en el empalme alternativo y la poliadenilación.
  • Explorar cómo el procesamiento de ARN influye en la función de las proteínas mecanicosensoriales.

Principales métodos:

  • Revisión de la literatura de estudios recientes.
  • Descripción de los agentes moleculares en la mecanotransducción.
  • Examen de las funciones de la proteína de unión al ARN bajo fuerza mecánica.
  • Resumen de los cambios de empalme genético y poliadenilación en respuesta a señales mecánicas.

Principales resultados:

  • Las vías de mecanotransducción están moduladas por mecanismos de procesamiento de ARN.
  • Las fuerzas mecánicas influyen en la actividad y función de las proteínas de unión al ARN.
  • Los genes que codifican las proteínas mecanosensoriales exhiben un empalme alternativo, afectando las funciones de la isoforma.
  • Las fuerzas mecánicas alteran los patrones globales alternativos de empalme y poliadenilación.

Conclusiones:

  • Hay una interacción significativa entre la detección mecánica celular y el procesamiento de ARN.
  • El empalme alternativo y la poliadenilación son nodos reguladores clave en la mecanotransducción.
  • Comprender estos vínculos es crucial para comprender las respuestas celulares a los entornos mecánicos.