Video Experimental Relacionado

Updated: Sep 9, 2025

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation
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Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation

Published on: June 21, 2021

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Un enfoque biofísico para estudiar las preferencias del sustrato de la cisteína oxidasa N-terminal

Karishma Patel1, Yannasittha Jiramongkol2, Mark D White3

  • 1School of Chemistry, The University of Sydney, Sydney, NSW, Australia; School of Life and Environmental Sciences, The University of Sydney, Sydney, NSW, Australia.

Methods in enzymology
|August 31, 2025
PubMed
Resumen

Las oxidasas de cisteína N-terminales (NCOs) actúan como sensores de oxígeno que regulan la estabilidad de las proteínas a través de la vía N-degron. Este estudio utiliza la resonancia plasmónica superficial (SPR) para analizar la unión de los NCO de mamíferos a sus sustratos, aclarando las interacciones enzima-sustrato.

Palabras clave:
Oficina de gestión de la calidadHipóxiaVía de N-degrónOxidasas de cisteína en el extremo NEl oxígenoModificación después de la traducciónTerminales de proteínasEnlace con el sustratoResonancia plasmónica de superficie

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