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Videos de Conceptos Relacionados

CRISPR01:59

CRISPR

52.9K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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Homologous Recombination02:31

Homologous Recombination

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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What is Genetic Engineering?00:49

What is Genetic Engineering?

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Overview
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Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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Updated: Sep 9, 2025

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
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Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

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Avances en la ingeniería de ADN a gran escala con el sistema CRISPR

Lee Wha Gwon1,2, Isabel Wen Badon3,4, Youngjeon Lee1,2

  • 1National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju, Republic of Korea.

Experimental & molecular medicine
|August 31, 2025
PubMed
Resumen
Este resumen es generado por máquina.

La inserción de ADN basada en repeticiones palindrómicas cortas con intervalos regulares agrupados (CRISPR) ofrece un método simplificado de un solo paso para la edición de genes in vivo. Esta tecnología avanzada combina CRISPR-Cas con recombinasas para una integración precisa y eficiente de ADN extraño en genes objetivo.

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Área de la Ciencia:

  • Biología molecular
  • Ingeniería genética
  • Biotecnología

Sus antecedentes:

  • Los métodos tradicionales de inserción de ADN a menudo requieren secuencias de reconocimiento de preingeniería o cruce genético.
  • Estos enfoques convencionales pueden ser ineficientes y complejos para la modificación génica in vivo.

Objetivo del estudio:

  • Proporcionar una visión general de los avances recientes en las tecnologías de inserción de ADN específico basadas en CRISPR.
  • Para discutir las posibles aplicaciones futuras de estas herramientas innovadoras de edición de genes.

Principales métodos:

  • Integración del módulo CRISPR-Cas con las enzimas de la recombinasa.
  • Desarrollo de la inserción de ADN in vivo en un solo paso en los genes objetivo.

Principales resultados:

  • La inserción de genes basada en CRISPR agiliza el proceso de ingeniería.
  • Consigue una inserción precisa y eficiente de ADN extraño en vivo.

Conclusiones:

  • La inserción de genes basada en CRISPR representa un avance significativo con respecto a los métodos tradicionales.
  • Esta tecnología es prometedora para diversas aplicaciones futuras en ingeniería genética y más allá.