Video Experimental Relacionado
Updated: Sep 9, 2025
11:30
Unravelling the Function of a Bacterial Effector from a Non-cultivable Plant Pathogen Using a Yeast Two-hybrid Screen
Published on: January 20, 2017
11.7K
La señalización del ácido jasmónico está dirigida por un efector fúngico Tin2-fold
Summia Gul1, Gabriel Mendoza-Rojas1, Max Heinen1
1Institute of Microbiology, Cluster of Excellence on Plant Sciences, Heinrich-Heine University Düsseldorf, 40225 Düsseldorf, Germany.
Journal of experimental botany
|September 2, 2025
Resumen
El efector fúngico Tue1 se dirige a la señalización del jasmonato de la planta mediante la interacción con las proteínas JAZ10. Esta interacción interrumpe las vías de jasmonato, afectando la defensa y el desarrollo de las plantas en respuesta a la infección por hongos.
Área de la Ciencia:
- Interacciones con patógenos vegetales
- Patología molecular de las plantas
- Biología de los efectores fúngicos
Sus antecedentes:
- La señalización de jasmonato integra las señales ambientales con el crecimiento y la defensa de las plantas.
- Los efectores patógenos a menudo se dirigen a las vías inmunitarias de las plantas, incluida la señalización del jasmonato.
- El hongo Thecaphora thlaspeos infecta las plantas de las Brassicaceae.
Objetivo del estudio:
- Para caracterizar la proteína efector fúngico Tue1 de Thecaphora thlaspeos.
- Investigar el mecanismo por el cual Tue1 interactúa con los componentes de señalización del jasmonato vegetal.
- Comprender el papel de Tue1 en las interacciones huésped-patógeno.
Principales métodos:
- Caracterización del efector fúngico Tue1.
- Análisis de la interacción de Tue1 con las proteínas del dominio ZIM 10 (JAZ10) del jasmonato vegetal mediante análisis guiado por la estructura.
- Investigando el efecto de la sobreexpresión de Tue1 en los niveles de jasmonato y la expresión génica en Arabidopsis thaliana.
- Estudio de la infección por T. thlaspeos en el Arabis hirsuta.
Principales resultados:
- El efector fúngico Tue1 se importa al núcleo de la planta a través de la vía de la importina-α.
- Tue1 interactúa directamente con las proteínas JAZ10 de las plantas anfitrionas y no anfitrionas.
- La interacción de Tue1 con JAZ10 sugiere un papel en la estabilización de JAZ10 o en la competencia con las proteínas que interactúan con JAZ10.
- La sobreexpresión de Tue1 conduce a niveles reducidos de jasmonato y a una expresión alterada de los genes sensibles al jasmonato.
- Tue1 pertenece a la familia de efectores conservados de Tin2 que se encuentran en los hongos smut.
Conclusiones:
- El efector fúngico Tue1 se dirige a la señalización del jasmonato de la planta mediante la interacción con los represores JAZ10.
- Esta interacción probablemente modula la defensa y el desarrollo de las plantas durante la infección por T. thlaspeos.
- La familia de efectores del pliegue 2, incluido el Tue1, representa un repertorio ampliado de factores de virulencia fúngica.
- Dirigirse a los repressores de JAZ puede ser una estrategia conservada o adquirida por los hongos porosos.
Palabras clave:
Arábis hirsuta y sus variedadesEl capófora thlaspeosCristalografía de rayos XProteína del efectorlocalización nuclearpatógeno perenneInteracción entre las plantas y los microbiosMás Videos Relacionados
07:55Luciferase Complementation Imaging Assay in Nicotiana benthamiana Leaves for Transiently Determining Protein-protein Interaction Dynamics
Published on: November 20, 2017
14.2K
11:16A Hydroponic Co-cultivation System for Simultaneous and Systematic Analysis of Plant/Microbe Molecular Interactions and Signaling
Published on: July 22, 2017
14.2K
Videos de Conceptos Relacionados
01:16TGF - β Signaling Pathway
7.6K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.6K
01:25Cell Signaling in Plants
5.7K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.7K
01:20The JAK-STAT Signaling Pathway
9.2K
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...
9.2K
03:14Notch Signaling Pathway
4.4K
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
4.4K
02:33Hedgehog Signaling Pathway
7.5K
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
7.5K
01:19Interactions Between Signaling Pathways
6.4K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
6.4K