La fosforilación de la α-catenina es sensible a la acomiosina y es necesaria para las funciones de la barrera epitelial a través de Afadin.
Contact us if these videos are not relevant.
Contact us if these videos are not relevant.
Ver abstracta en PubMed
Resumen
Este resumen es generado por máquina.La fosforilación alfa-catenina (α-cat), desencadenada por fuerzas mecánicas, lo prepara para unirse a Afadin. Esta interacción es crucial para el ensamblaje del complejo de unión apical y la función de la barrera epitelial, que afecta el desarrollo del cerebro.
Área De La Ciencia
- Biología celular
- Biología epitelial
- La biofísica
Sus Antecedentes
- El complejo de unión apical (AJC) mantiene la integridad de la barrera epitelial.
- La contractilidad de la actomyosina es vital para la estructura de la AJC, pero su regulación no está clara.
- La alfa-catenina (α-cat) es un componente mecanicamente sensible del zonula adherens (zAJ).
Objetivo Del Estudio
- Aclarar el papel de la fosforilación α-cat en el ensamblaje de la AJC y la función de la barrera epitelial.
- Identificar las partes mecanoquímicas de unión de α-cat.
- Investigar las consecuencias in vivo de la fosforilación alterada de α-cat.
Principales Métodos
- Ensayos de fosforilación in vitro para detectar la modificación α-cat dependiente de la fuerza.
- Identificación de los socios de unión α-cat mediante métodos bioquímicos.
- Análisis de un modelo de ratón con fosforilación α-gato interrumpida.
- Evaluación de la función de la barrera epitelial y la estructura de la AJC.
Principales Resultados
- La fosforilación dependiente de la fuerza de α-cat ocurre en su región de enlace no estructurada.
- La afadina se ha identificado como un socio mecanoquímico de unión del α-cat.
- La fosforilación α-cat y la unión de Afadin son esenciales para el enriquecimiento de zAJ y la función de barrera.
- La alteración de la fosforilación α-cat conduce a graves defectos en el desarrollo del cerebro postnatal en ratones.
Conclusiones
- Un modelo escalonado para la función α-cat en la integración de señales mecánicas y químicas en el zAJ.
- Las primas de fosforilación α-cat para la unión del efector, promoviendo el ensamblaje zAJ y la función de barrera.
- Esta vía es crítica para la homeostasis epitelial y el desarrollo embrionario, particularmente en el cerebro.
Contact us Si estos videos no son relevantes.
Contact us Si estos videos no son relevantes.
Videos de Conceptos Relacionados
The adherens junctions that anchor cells together are multi-protein complexes that dynamically adapt to mechanical stimuli such as tensile forces and shear stress. Mechanosensory proteins in these junctions can sense such mechanical stimuli and undergo a shift in their conformation, resulting in an altered function — a process called mechanotransduction.
α-Catenin as a Mechanosensory Protein
The α-catenin of adherens junctions is an allosteric protein with three VH (vinculin...
Catenins are characterized by multiple binding domains and dynamic structures that allow them to function as linker proteins in cell junction complexes. All catenins, except α-catenin, contain a characteristic protein sequence called the armadillo repeat and are therefore also called armadillo proteins.
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the...
Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
Strong contact points between adjacent cells anchor them to each other, forming tissues. Such anchoring junctions are of two types – adherens junctions and desmosomes. Adherens junctions are abundant in tissues such as epithelium and endothelium, forming a continuous zone of adhesion called the adhesion belt. In other tissues, such as heart muscle, they appear as clusters, linking the cells to produce coordinated heart muscle contraction.
Adherens Junctions are Dynamic
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
The straight or branched structure formation of actin filaments is controlled by nucleating proteins such as the formins and Arp2/3 complex. Formin-mediated assembly results in straight filaments, whereas Arp2/3 protein complex-mediated assembly results in branched actin filaments.
Arp2/3 Complex
Arp2/3 complex is a seven-subunit complex consisting of two proteins similar to actin- Arp2 and Arp3, and five other subunits that help keep Arp2 and Arp3 inactive. When required, the complex is...