Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Phosphoinositides and PIPs01:42

Phosphoinositides and PIPs

8.7K
Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
8.7K
IP3/DAG Signaling Pathway01:11

IP3/DAG Signaling Pathway

12.4K
Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the  phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and...
12.4K
Other Stress Responses in Bacteria01:30

Other Stress Responses in Bacteria

64
Bacteria have global regulatory systems that control several types of stress mechanisms. These include Pho regulon and the heat shock response, which are essential systems for environmental adaptation, such as nutrient limitation and proteotoxic stress. The Pho regulon and the heat shock response exemplify bacterial resilience, enabling rapid adaptation to fluctuating environmental conditions.Pho RegulonBacteria require phosphorus for essential cellular processes, including nucleic acid...
64
Regulation of the Unfolded Protein Response01:31

Regulation of the Unfolded Protein Response

2.6K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.6K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

3.9K
The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
3.9K
The Unfolded Protein Response01:37

The Unfolded Protein Response

5.1K
The ER is the hub of protein synthesis in a cell. It has robust systems to quality control protein folding and also for degradation of terminally misfolded proteins. Under normal conditions, a small proportion of misfolded proteins that cannot be salvaged need to be transported to the cytoplasm by the ER-associated degradation or ERAD pathways. However, if the ERAD cannot handle the misfolded proteins, the cell activates the unfolded protein response or UPR to adjust the protein folding...
5.1K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Applications of the Human Bone Marrow Organoid System to Study Hematopoiesis and Anemia Stress.

Tissue engineering. Part C, Methods·2026
Same author

BMX-001 acts as a selective chemoradioprotector in rectal cancer.

Redox biochemistry and chemistry·2026
Same author

A Rare Case of Mucopolysaccharidosis Presenting With Dysostosis Multiplex and Preserved Intelligence in a Seven-Year-Old Girl From Northeast India.

Cureus·2026
Same author

A generalizable Hi-C foundation model for chromatin architecture, single-cell and multiomics analysis across species.

Nature methods·2026
Same author

Predictive modeling of signal-responsive cis-elements in human red blood cell precursors.

Nucleic acids research·2026
Same author

Chromosome 5q deletion drives evolution of aneuploidy in myeloid neoplasms with complex karyotype.

Blood·2026

Video Experimental Relacionado

Updated: Sep 9, 2025

Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes
08:49

Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes

Published on: March 14, 2021

4.2K

Una proteína que interactúa con los fosfoinosídeos coordina las actividades precursoras del estrés

Pooja Roy, Blake A Rose, Suhita Ray

    bioRxiv : the preprint server for biology
    |September 2, 2025
    PubMed
    Resumen

    La proteína Samd14 es esencial para mantener la autofagia durante la producción de glóbulos rojos después de la anemia. Su interacción con PI3P equilibra la eritropoyesis, asegurando la formación de glóbulos rojos saludables.

    Más Videos Relacionados

    Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry
    08:07

    Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry

    Published on: July 26, 2019

    8.6K
    Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation
    10:52

    Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation

    Published on: January 6, 2016

    10.5K

    Videos de Experimentos Relacionados

    Last Updated: Sep 9, 2025

    Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes
    08:49

    Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes

    Published on: March 14, 2021

    4.2K
    Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry
    08:07

    Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry

    Published on: July 26, 2019

    8.6K
    Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation
    10:52

    Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation

    Published on: January 6, 2016

    10.5K

    Área de la Ciencia:

    • Biología celular
    • Hematología
    • Biología molecular

    Sus antecedentes:

    • La proteína Samd14 juega un papel crítico en la señalización celular y la supervivencia, particularmente en modelos de ratón de anemia aguda.
    • Posee una proteína de tapa de actina N-terminal (CP) y un dominio estéril de motivo alfa (SAM) C-terminal, que coordina las vías de señalización clave durante la diferenciación de precursores de glóbulos rojos.
    • Los procesos de autofagia se alteran significativamente en los precursores de glóbulos rojos durante la anemia aguda a medida que se acelera la eritropoyesis.

    Objetivo del estudio:

    • Investigar el papel de Samd14 en el mantenimiento de la autofagia equilibrada en los precursores de glóbulos rojos durante la anemia aguda.
    • Para aclarar la interacción entre Samd14 y el fosfatidilinositol 3-fosfato (PI3P) en la regulación de la eritropoyesis.
    • Determinar el impacto de la inhibición de VPS34 en la diferenciación eritroidea en presencia y ausencia de Samd14.

    Principales métodos:

    • Análisis de las firmas genéticas de la autofagia y los niveles de proteínas en los precursores de glóbulos rojos en condiciones de anemia aguda.
    • Investigación de la interacción del dominio SAM de Samd14 con PI3P mediante ensayos bioquímicos.
    • Utilizando un inhibidor de moléculas pequeñas (SAR405) de la clase III PI 3-cinasa VPS34 para evaluar su efecto sobre la diferenciación eritroide.
    • Comparación de los efectos de la inhibición de VPS34 en la diferenciación eritroidea en condiciones de tipo salvaje y deficiencia de Samd14.

    Principales resultados:

    • La expresión de Samd14 es necesaria para mantener la autofagia equilibrada en los precursores de glóbulos rojos después de una anemia aguda.
    • Samd14 interactúa directamente con PI3P, un componente lipídico de las membranas endosómicas y autofágicas, a través de su dominio SAM.
    • La inhibición de VPS34, la única quinasa para la producción de PI3P, bloqueó efectivamente la formación de glóbulos rojos.
    • La ausencia de Samd14 requirió dosis más altas de inhibición de VPS34 para impedir la diferenciación eritroidea, lo que indica una dependencia.

    Conclusiones:

    • Samd14 es crucial para regular la autofagia durante la respuesta al estrés de la anemia aguda, asegurando el mantenimiento de las células progenitoras.
    • La interacción entre Samd14 y PI3P es vital para equilibrar la eritropoyesis y producir glóbulos rojos maduros.
    • La regulación de la autofagia mediada por Samd14 es un mecanismo clave para mantener las poblaciones de células madre hematopoyéticas y garantizar la homeostasis de los glóbulos rojos.