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El propilparabeno afecta el desarrollo muscular en peces cebra a través de la vía PI3K-mTOR
Yong Huang1, Runhao Zhu2, Yan Lei3
1State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi 330000, China.
Ecotoxicology and environmental safety
|September 2, 2025
Resumen
La exposición al propilparabeno (PP) afecta el desarrollo y la función muscular del pez cebra inhibiendo los factores miogénicos clave y la vía PI3K-mTOR. La restauración de esta vía puede mitigar la toxicidad muscular inducida por los conservantes en las miopatías congénitas.
Área de la Ciencia:
- Biología muscular
- Toxicología
- Biología del desarrollo
Sus antecedentes:
- Las miopatías congénitas son trastornos musculares genéticos.
- Las toxinas ambientales pueden empeorar estas condiciones.
- La toxicidad muscular del propilparabeno (PP) es poco conocida.
Objetivo del estudio:
- Investigar los mecanismos de toxicidad muscular del PP.
- Utilice el modelo de pez cebra Tg (-1.9mylpfa: EGFP).
- Explorar el impacto de PP en el desarrollo muscular y el comportamiento.
Principales métodos:
- Imágenes de pez cebra y análisis de comportamiento.
- PCR cuantitativa (qPCR) y hibridación in situ.
- Evaluación de la vía PI3K-mTOR y los niveles de ROS.
Principales resultados:
- PP deterioró la fibra muscular y el desarrollo de células precursoras.
- Reducción de la actividad motora y el rendimiento de las larvas.
- PP inhibió la vía MyoD, Myf5, PI3K-mTOR y aumentó la ROS.
- Los activadores de mTOR rescataron la toxicidad inducida por PP.
Conclusiones:
- PP induce la toxicidad muscular a través de la inhibición de MyoD/ Myf5 y la supresión de PI3K-mTOR.
- Los conservantes ambientales pueden exacerbar los trastornos musculares genéticos.
- Dirigirse a la vía PI3K-mTOR ofrece un potencial terapéutico.
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