Video Experimental Relacionado
Updated: Sep 9, 2025
10:07
Isolation of Giant Lampbrush Chromosomes from Living Oocytes of Frogs and Salamanders
Published on: December 5, 2016
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Ensamblaje del genoma a nivel de cromosomas de la rana tirrena (Hyla sarda)
Josephine R Paris1,2, Roberta Bisconti3, Andrea Chiocchio4
1Department of Health, Life and Environmental Sciences, University of L'Aquila, Coppito, Italy.
Scientific data
|September 2, 2025
Resumen
Se creó un ensamblaje de genoma de alta calidad para la rana tirrena (Hyla sarda). Este recurso ayuda a comprender la base genética de los cambios fenotípicos durante su expansión evolutiva.
Área de la Ciencia:
- La genómica
- Biología evolutiva
- Investigaciones sobre anfibios
Sus antecedentes:
- La rana del árbol tirreno (Hyla sarda) exhibe variación fenotípica vinculada a la expansión del rango.
- La comprensión de los fundamentos genéticos de esta variación está limitada por la ausencia de un genoma de referencia.
Objetivo del estudio:
- Para ensamblar un genoma de referencia a nivel cromosómico para Hyla sarda.
- Proporcionar un recurso genómico para el estudio de la evolución y la conservación.
Principales métodos:
- Utilizó lecturas largas de PacBio HiFi, mapas ópticos Bionano y datos Hi-C para el ensamblaje del genoma.
- Utilizó canalizaciones bioinformáticas estándar para el ensamblaje del genoma y la anotación de genes.
Principales resultados:
- Se generó un ensamblaje de genoma a nivel cromosómico de 4,15 Gb con 13 cromosomas.
- Se obtuvieron puntuaciones de alta integridad (BUSCO 94,60%, k-mer 98,30%) y 22.847 genes codificadores de proteínas anotados.
- Los elementos repetitivos constituyen aproximadamente el 75% del genoma ensamblado.
Conclusiones:
- El ensamblaje del genoma de Hyla sarda de alta calidad es un recurso crucial para la genómica evolutiva y de conservación.
- Facilita la investigación de las correlaciones genotipo-fenotipo durante los cambios de rango impulsados por los glaciares.
- Permite futuros estudios genómicos de poblaciones para los esfuerzos de conservación de Hyla sarda.
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