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Retrovirus ovino exógeno (JSRV) de la cepa de Mongolia Interior: caracterización de todo el genoma y envasado de

Pei Zhang^1,2, Yu Wang^1,2, Xujie Duan^1,2

¹College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, China.

Frontiers in veterinary science

|September 3, 2025

Resumen

Este estudio amplificó con éxito el genoma completo del retrovirus exógeno de Jaagsiekte (exJSRV) en Mongolia Interior. Esto proporciona datos cruciales para comprender el adenocarcinoma pulmonar ovino (AOP) y desarrollar estrategias de control.

Área de la Ciencia:

Virología veterinaria
Los retrovirus
La genómica

Sus antecedentes:

El adenocarcinoma pulmonar ovino (APO) es un tumor pulmonar contagioso en las ovejas, causado por el retrovirus ovino exógeno Jaagsiekte (exJSRV).
La información genómica del exJSRV es esencial para desarrollar estrategias efectivas de prevención y control de la OPA.
Hubo una falta de datos genómicos exJSRV específicos para Mongolia Interior.

Objetivo del estudio:

Establecer una técnica específica para la amplificación genómica del exJSRV en Mongolia Interior.
Obtener el genoma completo de exJSRV de esta región.
Establecer las bases para el cribado de objetivos antivirales y la investigación de la patogénesis de la OPA.

Principales métodos:

Purificación de viriones mediante PCR heminestado con U3 (hn-PCR) y centrifugación por gradiente de densidad.
Diseño de primers de transcripción inversa específicos dirigidos a regiones de baja identidad.
Aplicación de la PCR de fragmento largo y la tecnología 3'RACE para la amplificación del genoma completo.

Principales resultados:

Se obtuvo con éxito el genoma completo de exJSRV de Mongolia Interior.
Las características moleculares revelaron una alta identidad (98,8%) con el JSRV-C1 chino, distinto de los aislados extranjeros y el enJSRV.
El análisis filogenético colocó el aislado de Mongolia Interior (NMJS12) en el mismo clado que exJSRV-C1.
Se construyó un plásmido de expresión eucariota de genoma completo, que permite el empaquetado de partículas virales en células 293T.

Conclusiones:

Este estudio informa la primera secuencia completa del genoma de exJSRV de Mongolia Interior, China.
Los hallazgos proporcionan datos genómicos críticos para la investigación de OPA.
Este trabajo apoya el desarrollo de objetivos antivirales y mejora la comprensión de la patogénesis de la OPA.

Palabras clave:

Retrovirus endógeno de las ovejas Jaagsiekte Retrovirus exógeno de las ovejas Jaagsiekte secuenciación genómica Adenocarcinoma pulmonar ovino Análisis filogenético partículas de virus

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