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Perfiles comparativos de fosfolípidos y esfingolípidos durante la infección aguda

Tao Liu¹, Hong Jia², Hui Dong³

¹College of Animal Science and Technology, Xinyang Agriculture and Forestry University, Xinyang 464000, China.

Microbial pathogenesis

|September 3, 2025

Resumen

Este estudio revela los perfiles lipídicos en el bazo y la bolsa de Fabricius durante las infecciones agudas, identificando posibles biomarcadores para el virus del síndrome reproductivo y respiratorio porcino, el virus de la diarrea epidémica porcina, el virus de la fiebre porcina africana y la Eimeria tenella. Estos hallazgos avanzan en la comprensión de la biología de los lípidos de los órganos linfoides y la intervención contra las infecciones.

Área de la Ciencia:

Patología veterinaria
La lipidomía
Investigación de enfermedades infecciosas

Sus antecedentes:

Los perfiles lipídicos de los órganos linfoides clave, el bazo y la bolsa de Fabricius (BF), durante las infecciones agudas no se conocen bien.
Las infecciones agudas en los cerdos plantean desafíos significativos para la salud animal y las economías agrícolas.

Objetivo del estudio:

Investigar y caracterizar los perfiles lipídicos del bazo de los cerdos y de la BF durante las infecciones virales y parasitarias agudas.
Identificar posibles biomarcadores lipídicos asociados con patógenos específicos como el PRRSV, el PEDV, el ASFV y la Eimeria tenella.

Principales métodos:

Desarrollo de modelos de infección aguda para el virus del síndrome respiratorio y reproductivo porcino (PRRSV), el virus de la diarrea epidémica porcina (PEDV) y la Eimeria tenella (ET).
Recolección y examen de muestras de bazo, incluidas las con y sin infección por el virus de la peste porcina africana (PPA).
Análisis lipidómico semicantitativo y no dirigido mediante cromatografía líquida y espectrometría de masas en tándem por electrospray (LC-MS-MS).

Principales resultados:

Se observaron lesiones microscópicas características en el bazo y la fibra ósea.
Se identificaron perfiles lipídicos distintos para cada modelo de infección.
Se propusieron biomarcadores potenciales para el PRRSV, el PEDV, el ASFV y el ET en el bazo y/o la fibrilación bucal.

Conclusiones:

El perfil lipídico del bazo y la bolsa de Fabricius proporciona información valiosa sobre la biología lipídica de los órganos linfoides durante las infecciones agudas.
Los biomarcadores lipídicos identificados pueden contribuir a una mejor comprensión y a posibles estrategias de intervención para las enfermedades porcinas.

Palabras clave:

Infección aguda LC-MS-MS (en inglés)Los fosfolípidos Esfingolipídico El bazo la bolsa de Fabricio

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