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El formato intracelular modula un interruptor de movilidad-invasión en Salmonella Typhimurium
Debapriya Mukherjee1, Salik Noor2, Tamoghna Mukherjee2
1Department of Microbiology and Cell Biology, Division of Biological Sciences, Indian Institute of Science, Bangalore, India.
PLoS pathogens
|September 4, 2025
Resumen
El formato intracelular es crucial para la virulencia de Salmonella Typhimurium (STM), regulando el equilibrio del pH y la expresión génica. El formato agotador interrumpe la flagelación y promueve la invasión, destacando su papel en la patogénesis bacteriana.
Área de la Ciencia:
- Microbiología
- Patogénesis bacteriana
- Biología molecular
Sus antecedentes:
- Los ácidos grasos de cadena corta (SCFA) derivados del huésped son vitales para la virulencia de Salmonella Typhimurium (STM).
- El papel del formato intracelular en la patogénesis del STM no se comprende bien.
Objetivo del estudio:
- Investigar la función del grupo de formato intracelular en la virulencia del STM.
- Aclarar los mecanismos reguladores que vinculan el metabolismo del formato con la invasión del STM.
Principales métodos:
- Eliminación del gen piruvato-formato liasa (pflB) para agotar el formato intracelular.
- Análisis de la flagelación, la expresión génica de la isla-1 (SPI-1) (hilA, prgH), el pH intracelular y la integridad de la membrana.
- Investigar el papel de las vías RpoE y CsrA/csrB.
Principales resultados:
- La deleción de pflB redujo la flagelación y aumentó la expresión del gen SPI-1.
- Este cambio estuvo relacionado con un pH intracelular elevado y daño a la membrana.
- La reposición del formato restauró la modulación de la virulencia en STM ΔpflB.
- La RpoE y la vía CsrA/csrB regulan esta transición.
Conclusiones:
- El formato intracelular es crítico para mantener la homeostasis del pH y coordinar la expresión del gen de virulencia en el STM.
- La expresión de pflB de ajuste fino es esencial para una invasión óptima del STM a través de diferentes regiones intestinales.
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