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Expresión conservada y distinta de ARN circulares en los virus de la vacuna de la enfermedad de Marek de uso
Camille Ponsard1, Alexis S Chasseur1,2, Pierre Lombard1
1Namur Research Institute for Life Sciences (NARILIS), Integrated Veterinary Research Unit (URVI), University of Namur, Namur, Belgium.
The Journal of general virology
|September 5, 2025
Resumen
Los ARN circulares (circRNA) se encuentran en el virus de la enfermedad de Marek (GaHV-2) y en los alfaherpesvirus aviares relacionados. Estos circRNA virales pueden desempeñar un papel en el ciclo de vida viral y la propagación.
Área de la Ciencia:
- Virología
- Biología molecular
- Biología del ARN
Sus antecedentes:
- Los ARN circulares (circRNA) son cada vez más reconocidos por su papel en varios procesos biológicos.
- La expresión aberrante de circRNA está relacionada con el cáncer, y su papel en las infecciones virales es un área de investigación emergente.
- El virus de la enfermedad de Marek (Gallid alfa 2, GaHV-2) es un patógeno significativo en aves de corral, con circRNA identificados previamente en cepas virulentas.
Objetivo del estudio:
- Para identificar los nuevos loci circRNA virales en las distintas etapas de la infección por GaHV-2.
- Para investigar las firmas virales de circRNA en las vacunas de uso común contra la enfermedad de Marek, incluidas las avirulentas GaHV-2, GaHV-3 y MeHV-1.
- Caracterizar el papel de los circRNA en el ciclo de vida viral y la propagación de los alfaherpesvirus aviares.
Principales métodos:
- Catalogación de los circRNA de los genes de virulencia y las transcripciones asociadas a la latencia (LAT) en GaHV-2.
- Identificación de la expresión del circRNA viral de los loci conservados, incluidas las regiones antisenso de ICP4 y las proteínas de envasado de ADN que codifican, en diferentes virus del alfaherpes aviar.
- Análisis comparativo de circRNAs de cepas virulentas y avirulentas de GaHV-2, centrado en el locus meq.
Principales resultados:
- Se descubrieron nuevos loci circRNA virales en la infección por GaHV-2.
- Se identificó un patrón de expresión circRNA viral conservado en GaHV-2, GaHV-3 y MeHV-1, originado en regiones antisense a ICP4 y codificando proteínas de envasado de ADN.
- Se observaron niveles distintos de expresión de circRNA en el locus meq entre las cepas virulentas y avirulentas de GaHV-2.
Conclusiones:
- Existe un patrón conservado de circRNAs derivados del virus en los alfaherpesvirus aviares relacionados.
- Es probable que estos circARN virales conservados contribuyan a la finalización del ciclo viral y faciliten la propagación viral.
- Las investigaciones adicionales sobre los circRNA virales podrían revelar nuevos objetivos para controlar la enfermedad de Marek y las infecciones virales relacionadas.
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