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La activación dependiente de la fosforilación de MAP4K1/2 por OST1 media el cierre estomático inducido por ABA en

Dongxue Tang¹, Dan Pei², Meixiang Zhang³

¹State Key Laboratory of Plant Environmental Resilience, Frontiers Science Center for Molecular Design Breeding, College of Biological Sciences, China Agricultural University, Beijing, 100193, China.

Journal of integrative plant biology

|September 5, 2025

Resumen

La proteína quinasa activada por mitógeno 1 (MAP4K1) y MAP4K2 son reguladores clave del equilibrio hídrico de las plantas. Los OST1 activados por ABA fosforilan MAP4K1/2, que luego regulan el cierre estomático para la tolerancia a la sequía.

Área de la Ciencia:

Fisiología vegetal
Biología molecular
La bioquímica

Sus antecedentes:

La regulación estomatal es vital para la homeostasis del agua de las plantas y el intercambio de gases.
Los estomas abiertos 1 (OST1) son un regulador conocido en las vías de señalización del ácido abscísico (ABA) que controlan el movimiento estomático.

Objetivo del estudio:

Identificar nuevos reguladores en el cierre estomático mediado por ABA.
Para aclarar la cascada de señalización aguas abajo de OST1 en respuesta a ABA.

Principales métodos:

Análisis del sitio de fosforilación de MAP4K1/2 por OST1.
Ensayos de fosforilación in vitro y in vivo.
Caracterización funcional de MAP4K1/2 utilizando el análisis de mutantes y las variantes fosfomiméticas/no fosforilables.

Principales resultados:

MAP4K1 y MAP4K2 fueron identificados como reguladores positivos del cierre estomático inducido por ABA.
OST1 fosforila MAP4K1/2 en residuos específicos, activándolos.
El MAP4K1 activado fosforila la membrana plasmática H+-ATPasa 2 (AHA2) y el asociado al canal aniónico lento 1 (SLAC1).
El fosfomimético MAP4K1 restauró la tolerancia a la sequía en los mutantes map4k1map4k2.

Conclusiones:

Se descubrió un nuevo módulo de señalización OST1-MAP4K1/2-AHA2/SLAC1 que controla el cierre estomático.
Esta vía es crucial para las relaciones de agua de las plantas mediadas por ABA y la adaptación al estrés.
Los hallazgos profundizan en la comprensión de las cascadas de fosforilación en las respuestas al estrés de las plantas.

Palabras clave:

AHA2 (en inglés)MAP4K1 y 2 Se incluyen los siguientes elementos:SLAC1 y sus derivados movimientos estomales

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