Los leucocitos usan túneles de la membrana endotelial para extravasar la vasculatura
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Ver abstracta en PubMed
Resumen
Este resumen es generado por máquina.Las células endoteliales forman túneles de membrana, no se desconectan, durante la extravasación de neutrófilos. Los neutrófilos utilizan estos túneles regulados por actina para el paso, revelando una nueva arquitectura 3D para la transmigración de leucocitos.
Área De La Ciencia
- Biología celular
- Inmunología
- La biofísica
Sus Antecedentes
- La extravasación de leucocitos es crucial para la respuesta inmune.
- Anteriormente se pensaba que las uniones de células endoteliales se desconectaban por completo durante la transmigración.
Objetivo Del Estudio
- Para investigar la arquitectura 3D de las uniones de células endoteliales durante la transmigración de neutrófilos.
- Para aclarar el papel de las interacciones de las células endoteliales en la extravasación de leucocitos.
Principales Métodos
- Imágenes en vivo en 3D de alta resolución.
- Análisis de la dinámica de las uniones de las células endoteliales.
- Investigó el papel de la polimerización de la actina, el PECAM-1 y la VE-cadherina.
Principales Resultados
- Las células endoteliales muestran superposiciones parciales de la membrana más allá de la VE-cadera.
- La polimerización de la actina regula estas superposiciones, que no requieren PECAM-1 o VE-cadherina.
- Los neutrófilos crean y utilizan túneles de membrana formados por la superposición de células endoteliales para la transmigración.
Conclusiones
- Las células endoteliales forman activamente túneles de membrana para facilitar el paso de los neutrófilos.
- Esto desafía la noción de simple desconexión de células endoteliales.
- Define una nueva arquitectura 3D multicelular para la transmigración paracelular.
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