Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Intracellular Movement of Viruses and Bacteria01:10

Intracellular Movement of Viruses and Bacteria

3.4K
Intracellular bacteria and viruses often comprise a group of highly infectious pathogens that can cause several diseases. Bacterial pathogens include those belonging to the genus Rickettsia responsible for conditions such as rocky mountain spotted fever and the Mediterranean spotted fever; Chlamydia, a genus responsible for a sexually transmitted disease; Coxiella burnetii, an agent responsible for Q fever. Viral pathogens include vaccinia—a poxvirus, and herpes simplex virus—a...
3.4K
Bacterial Translocation and Protein Secretion01:26

Bacterial Translocation and Protein Secretion

513
Bacterial protein secretion involves translocation systems to ensure proteins reach their designated locations, including the plasma membrane, periplasm, outer membrane, or the external environment. These translocation systems are vital for bacterial physiology, supporting processes like membrane assembly, enzymatic activity in the periplasm, and interactions with the external environment. The division of labor between Sec and Tat pathways ensures efficiency in handling proteins with diverse...
513
Receptor-mediated Endocytosis01:20

Receptor-mediated Endocytosis

7.5K
Receptor-mediated endocytosis is when bulk amounts of specific molecules are imported into a cell after binding to cell surface receptors. The molecules bound to these receptors are taken into the cell through inward folding of the cell surface membrane, which is eventually pinched off into a vesicle within the cell. Structural proteins, such as clathrin, coat the budding vesicle.
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...
7.5K
Defense Against Bacterial Pathogens01:31

Defense Against Bacterial Pathogens

2.6K
The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
2.6K
Cancer Cell Migration through Invadopodia01:35

Cancer Cell Migration through Invadopodia

3.2K
Invadosome is a broad category of cell surface structures with proteolytic activity that  degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However,...
3.2K
Diversity of Protists I01:15

Diversity of Protists I

841
Excavata is a diverse group of protists that includes both chemoorganotrophic and phototrophic species, with some thriving in anaerobic environments. Among the key groups within Excavata are diplomonads and parabasalids, which are flagellated protists that lack mitochondria and chloroplasts. These microorganisms typically inhabit anoxic environments, such as the intestines of animals, where they exist either symbiotically or as parasites, relying on fermentation for energy production. Some...
841

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Mapping the dialogue: Decoding alveolar stem-niche interactions.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

TREM1 signaling amplifies neutrophil-mediated inflammation in inflammatory bowel disease.

Nature communications·2026
Same author

Surveying the roles of NINJ1 in plasma membrane rupture.

Nature structural & molecular biology·2026
Same author

Architecture and function of holocentric CENP-A-independent inner kinetochores.

Science advances·2026
Same author

Direct RNA Sequencing reveals epitranscriptomic regulation of brain cells and Alzheimer's Disease pathology.

bioRxiv : the preprint server for biology·2026
Same author

Outer membrane vesicles hijack TIM-1 for cellular uptake.

PLoS pathogens·2026

Video Experimental Relacionado

Updated: Jan 14, 2026

Assessment of Intestinal Transcytosis of Neonatal Escherichia coli Bacteremia Isolates
08:32

Assessment of Intestinal Transcytosis of Neonatal Escherichia coli Bacteremia Isolates

Published on: February 17, 2023

1.7K

Las bacterias enteropatógenas evaden la extrusión de células epiteliales impulsadas por ROCK

Giovanni Luchetti1, Marin V Miner2, Rachael M Peterson2

  • 1Department of Discovery Oncology, Genentech, South San Francisco, CA, USA. luchettg@gene.com.

Nature
|October 22, 2025
PubMed
Resumen

El factor de virulencia NleL de Escherichia coli previene la extrusión de las células epiteliales intestinales mediante la degradación de ROCK1/2. Este mecanismo permite a los patógenos evadir las defensas del huésped, destacando una carrera armamentista huésped-patógeno.

Más Videos Relacionados

Analysis of the Epithelial Damage Produced by Entamoeba histolytica Infection
11:49

Analysis of the Epithelial Damage Produced by Entamoeba histolytica Infection

Published on: June 12, 2014

19.1K
Author Spotlight: Advancements in Understanding and Combatting Shigella Infections
04:56

Author Spotlight: Advancements in Understanding and Combatting Shigella Infections

Published on: February 9, 2024

1.5K

Videos de Experimentos Relacionados

Last Updated: Jan 14, 2026

Assessment of Intestinal Transcytosis of Neonatal Escherichia coli Bacteremia Isolates
08:32

Assessment of Intestinal Transcytosis of Neonatal Escherichia coli Bacteremia Isolates

Published on: February 17, 2023

1.7K
Analysis of the Epithelial Damage Produced by Entamoeba histolytica Infection
11:49

Analysis of the Epithelial Damage Produced by Entamoeba histolytica Infection

Published on: June 12, 2014

19.1K
Author Spotlight: Advancements in Understanding and Combatting Shigella Infections
04:56

Author Spotlight: Advancements in Understanding and Combatting Shigella Infections

Published on: February 9, 2024

1.5K

Área de la Ciencia:

  • Microbiología
  • Inmunología
  • Biología celular

Sus antecedentes:

  • Los patógenos usan factores de virulencia para evadir los programas de muerte celular del huésped como la apoptosis.
  • La extrusión de células epiteliales intestinales (CEI) es un mecanismo de defensa del huésped para eliminar las células infectadas.
  • Los mecanismos patógenos dirigidos a los cambios citoesqueléticos para la extrusión de IEC eran desconocidos anteriormente.

Objetivo del estudio:

  • Investigar el papel de la ubiquitina ligasa NleL de Escherichia coli en la inhibición de la extrusión de las células epiteliales intestinales.
  • Identificar los objetivos del huésped de NleL implicados en la regulación de la extrusión celular.

Principales métodos:

  • Se ha investigado la función de E. coli NleL en IEC cultivados y en modelos de ratón.
  • Utilizó la deleción genética de NleL, ROCK1 y ROCK2.
  • Se analizó la extrusión IEC inducida por inflamatorios y la colonización bacteriana.

Principales resultados:

  • E. coli NleL inhibe la extrusión IEC al dirigirse a la caspasa-4, ROCK1 y ROCK2 para su degradación.
  • La eliminación de ROCK1 y ROCK2 en IECs redujo la extrusión inducida por el inflamatorio.
  • Citrobacter rodentium con deficiencia de NleL mostró una reducción de la colonización debido al aumento de la extrusión IEC.

Conclusiones:

  • NleL es un factor de virulencia que suprime la defensa de la barrera epitelial del huésped inhibiendo la extrusión de IEC.
  • ROCK1 y ROCK2 son reguladores clave de la extrusión IEC dirigidos por NleL.
  • Este estudio revela una nueva interacción huésped-patógeno que implica la regulación de la barrera epitelial.