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Inserción de ADN programable en las bacterias intestinales nativas

Amandine Maire¹, David Bikard¹

¹Institut Pasteur, Université Paris Cité, CNRS UMR 3525, Synthetic Biology, Paris, France.

Science (New York, N.Y.)

|November 13, 2025

Resumen

Los científicos desarrollaron un nuevo método de edición de genes para alterar con precisión las bacterias que residen en el intestino del ratón. Este avance permite modificaciones genéticas dirigidas dentro del complejo entorno del microbioma intestinal.

Área de la Ciencia:

Microbiología
La genética
Gastroenterología

Sus antecedentes:

El microbioma intestinal juega un papel crucial en la salud del huésped.
La modificación específica de las bacterias intestinales es un desafío.
Las tecnologías de edición genética ofrecen soluciones potenciales.

Objetivo del estudio:

Desarrollar y demostrar un sistema de edición de genes para modificar bacterias dentro del intestino del ratón.
Investigar la viabilidad de la manipulación genética bacteriana in vivo.

Principales métodos:

Utilizó un sistema de edición de genes basado en CRISPR entregado al tracto gastrointestinal del ratón.
Las bacterias diseñadas con modificaciones genéticas específicas.
Evaluó la eficiencia y la especificidad de la edición de genes en bacterias intestinales.

Principales resultados:

Se demostró con éxito la edición de genes dirigidos en bacterias dentro del intestino del ratón.
Confirmó la presencia y estabilidad de las modificaciones genéticas.
Mostró el potencial para la ingeniería del microbioma intestinal.

Conclusiones:

Un nuevo enfoque de edición de genes permite la modificación de bacterias dentro del intestino del ratón.
Esta tecnología abre nuevas vías para la investigación del microbioma y las intervenciones terapéuticas.

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