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Una caja de herramientas modular de ARN polimerasa T7 que vincula la detección selectiva de miARN con la

Maria Vonk-de Roy¹, Andreas Walther¹

¹Life-Like Materials and Systems, University of Mainz, Duesbergweg 10-14, 55128 Mainz, Germany.

Journal of the American Chemical Society

|November 19, 2025

Resumen

No abstract available in PubMed .

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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