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Updated: Jan 8, 2026

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OsPUB36 preferencial de raíz modula los niveles de ROS y regula el desarrollo de la raíz

Sunok Moon¹, Yo-Han Yoo², Ha Eun Park¹

¹Graduate School of Green Bio-Science & Crop Biotech Institute, Kyung Hee University, Yongin, 17104, Republic of Korea.

Plant cell reports

|December 12, 2025

Abstracto

Key Message:

OsPUB36, a root-preferential E3 ubiquitin ligase, modulates reactive oxygen species homeostasis and regulates rice root development, affecting primary root and root hair growth. Root development is tightly regulated by spatial gradients of reactive oxygen species (ROS), which coordinate transitions from cell division to elongation and differentiation. Through phylogenetic meta-analysis, we identified a root-preferential subclass of class III PUB E3 ubiquitin ligases (OsPUB31-OsPUB37). Among them, OsPUB36 was selected as a representative gene for functional characterization. Overexpressing OsPUB36, an endoplasmic reticulum (ER)-localized protein, resulted in shortened primary roots and elongated root hairs. RNA-seq and proteomic analysis of root hairs from plants overexpressing OsPUB36 revealed significant upregulation of class III peroxidases, key enzymes involved in ROS homeostasis. Histochemical staining confirmed enhanced levels of hydrogen peroxide (H₂O₂) in root hair. However, the loss-of-function mutants (ospub36 and ospub35 ospub36) displayed no obvious phenotypes, suggesting functional redundancy within these gene subclasses. While yeast two-hybrid screen did not identify direct targets related to ROS or root development, RNA-seq, proteomic, and histochemical analyses suggest that OsPUB36 and other class III PUBs modulate ROS homeostasis.

Palabras clave:

OsPUB36 ROS Raíz Pelos de raíz

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