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Co-infección mutualista-patógena: modulación de las firmas de fosfoinositidos en las interfaces intracelulares del

Alex Guyon¹, Theresa Staps², Lyne Badot³

¹Sainsbury Laboratory, University of Cambridge, Cambridge, UK; Department of Plant Sciences, University of Cambridge, Cambridge, UK.

|December 12, 2025

Resumen

Las membranas de las raíces de las plantas cambian sus firmas de fosfoinositidos (PI) durante las interacciones con microbios. La co-infección remodeló dinámicamente estas membranas del huésped, alterando las interacciones entre hongos patógenos y mutualistas.

Área de la Ciencia:

Biología vegetal; Microbiología; Biología celular

Sus antecedentes:

Las membranas del huésped que rodean a los microbios intracelulares son interfaces críticas que influyen en las interacciones huésped-microbio.
Existen identidades distintas de fosfoinositidos en las interfaces patógenas frente a las mutualistas, pero su modulación dinámica durante la co-infección no está clara.

Objetivo del estudio:

Investigar si las firmas de fosfoinositidos en las interfaces huésped-microbio se modulan durante la co-infección.
Determinar si estas modulaciones influyen en el resultado de las interacciones huésped-microbio.

Principales métodos:

Se generaron plantas de Nicotiana benthamiana que expresaban biosensores para el fosfatidilinositol 4-fosfato (PI4P) y el fosfatidilinositol 4,5-bisfosfato (PI(4,5)P2).
Se imaginó la colonización de raíces por el oomiceto patógeno Phytophthora palmivora y el hongo mutualista Funneliformis mosseae.
Se analizó la distribución de fosfoinositidos en las interfaces huésped-microbio durante interacciones binarias y de co-infección.

Principales resultados:

Se observaron patrones distintos de fosfoinositidos en interacciones binarias: el PI(4,5)P2 se enriqueció en los extremos de las estructuras mutualistas y se distribuyó uniformemente alrededor de las estructuras del patógeno, mientras que el PI4P estuvo ausente en las interfaces del patógeno pero presente en las interfaces mutualistas.
La co-infección alteró significativamente la identidad de la membrana del huésped, induciendo el reclutamiento de PI4P en las haustorias del patógeno.
Esta remodelación mejoró la resistencia de la planta al patógeno P. palmivora.

Conclusiones:

Las firmas de fosfoinositidos distinguen eficazmente las interfaces patógenas y mutualistas huésped-microbio.
Las identidades de fosfoinositidos de la membrana del huésped se remodelan dinámicamente durante la co-infección.
La remodelación dinámica de los fosfoinositidos probablemente juega un papel crucial en la determinación del resultado de las interacciones huésped-microbio.

Palabras clave:

microbiología plantas Nicotiana benthamiana Phytophthora palmivora simbiosis micorrízica arbuscular membrana extrahaustorial membrana periarbuscular fosfolípidos patógeno vegetal

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