Video Experimental Relacionado

Updated: Jan 8, 2026

Perforated Patch-clamp Recording of Mouse Olfactory Sensory Neurons in Intact Neuroepithelium: Functional Analysis of Neurons Expressing an Identified Odorant Receptor
10:16

Perforated Patch-clamp Recording of Mouse Olfactory Sensory Neurons in Intact Neuroepithelium: Functional Analysis of Neurons Expressing an Identified Odorant Receptor

Published on: July 13, 2015

27.3K

Cultivos Primarios de Neuronas Olfativas de Polilla

Philippe Lucas1, Christelle Monsempès2

  • 1Institute of Ecology and Environmental Sciences of Paris, Institut National de Recherche pour l'Agriculture l'Alimentation et l'Environnement (INRAE), Sorbonne Université, Centre national de la recherche scientifique (CNRS), Institut de Recherche pour le Développement (IRD), Université Paris-Est Créteil (UPEC), Université de Paris; philippe.lucas@inrae.fr.

Journal of visualized experiments : JoVE
|December 15, 2025
PubMed
Resumen

Este estudio presenta un nuevo protocolo para el cultivo de neuronas receptoras olfativas (ORN) de insectos a partir de pupas de polilla. Este método permite registros de patch-clamp para un análisis detallado de las vías de señalización de las ORN.

Más Videos Relacionados

An Effective Manual Deboning Method To Prepare Intact Mouse Nasal Tissue With Preserved Anatomical Organization15:40

An Effective Manual Deboning Method To Prepare Intact Mouse Nasal Tissue With Preserved Anatomical Organization

Published on: August 10, 2013

37.7K
Quadruple Immunostaining of the Olfactory Bulb for Visualization of Olfactory Sensory Axon Molecular Identity Codes06:32

Quadruple Immunostaining of the Olfactory Bulb for Visualization of Olfactory Sensory Axon Molecular Identity Codes

Published on: June 5, 2017

7.5K

Videos de Conceptos Relacionados

Olfactory Receptors: Location and Structure01:03

Olfactory Receptors: Location and Structure

The process of olfaction, also known as the sense of smell, is a sophisticated chemical response system. The specialized sensory neurons that facilitate this process, known as olfactory receptor neurons, are situated in an upper segment of the nasal cavity, known as the olfactory epithelium. Olfactory sensory neurons are bipolar, with their dendrites extending from the epithelium's apex into the mucus that lines the nasal cavity. Airborne molecules, when inhaled, traverse the olfactory...
11.1K
Olfaction01:25

Olfaction

The sense of smell is achieved through the activities of the olfactory system. It starts when an airborne odorant enters the nasal cavity and reaches olfactory epithelium (OE). The OE is protected by a thin layer of mucus, which also serves the purpose of dissolving more complex compounds into simpler chemical odorants. The size of the OE and the density of sensory neurons varies among species; in humans, the OE is only about 9-10 cm2.
The olfactory receptors are embedded in the cilia of the...
48.0K