Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Initiation of Translation02:33

Initiation of Translation

38.2K
Initiating translation is complex because it involves multiple molecules. Initiator tRNA, ribosomal subunits, and eukaryotic initiation factors (eIFs) are all required to assemble on the initiation codon of mRNA. This process consists of several steps that are mediated by different eIFs.
First, the initiator tRNA must be selected from the pool of elongator tRNAs by eukaryotic initiation factor 2 (eIF2). The initiator tRNA (Met-tRNAi) has conserved sequence elements including modified bases at...
38.2K
Initiation of Translation02:33

Initiation of Translation

7.8K
7.8K
Translation in Prokaryotes01:29

Translation in Prokaryotes

1.3K
Prokaryote translation is a complex, highly coordinated process that converts genetic information from mRNA into functional proteins. It involves three stages: initiation, elongation, and termination, each facilitated by specific molecular components.Initiation of TranslationThe process begins with the assembly of the ribosomal subunits and initiation factors on the mRNA. In bacteria, the 30S ribosomal subunit recognizes the Shine-Dalgarno sequence in the mRNA, a conserved region upstream of...
1.3K
Improving Translational Accuracy02:07

Improving Translational Accuracy

14.0K
Base complementarity between the three base pairs of mRNA codon and the tRNA anticodon is not a failsafe mechanism. Inaccuracies can range from a single mismatch to no correct base pairing at all. The free energy difference between the correct and nearly correct base pairs can be as small as 3 kcal/ mol. With complementarity being the only proofreading step, the estimated error frequency would be one wrong amino acid in every 100 amino acids incorporated. However, error frequencies observed in...
14.0K
Leaky Scanning02:28

Leaky Scanning

5.6K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.6K
General Transcription Factors01:30

General Transcription Factors

6.6K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
6.6K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

PKCβII Activation Promotes Membrane-Proximal Enrichment of Ribosome-Bound RACK1.

International journal of molecular sciences·2026
Same author

Dual role of 3' UTR length in modulating translation termination efficiency.

RNA (New York, N.Y.)·2026
Same author

Eukaryotic initiation factor eIF3 facilitates loading of eukaryotic release factor eRF1 or suppressor tRNA to the ribosome.

Nucleic acids research·2026
Same author

A Duality of Function: An Integrative Model of RACK1 as a Switch Between Translational and Signaling Hubs.

International journal of molecular sciences·2025
Same author

Eukaryotic initiation factors eIF4F and eIF4B promote translation termination upon closed-loop formation.

Nucleic acids research·2025
Same author

Functional Activity of Isoform 2 of Human eRF1.

International journal of molecular sciences·2024

Video Experimental Relacionado

Updated: Jan 8, 2026

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells
08:47

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells

Published on: May 1, 2020

3.3K

Factor de iniciación de la traducción eucariota 4F: propiedades funcionales y papel fisiológico

Ekaterina Shuvalova1, Walaa Al Sheikh1,2, Alexey Shuvalov1

  • 1Engelhardt Institute of Molecular Biology, the Russian Academy of Sciences, Moscow, 119991, Russia.

Nucleic acids research
|December 17, 2025
PubMed
Resumen

El complejo del factor de iniciación eucariota 4F (eIF4F) es crucial para la iniciación de la traducción de proteínas. Esta revisión explora eIF4F

Palabras clave:
factor de iniciación eucariota 4Ftraducciónregulación génicabiología celularenfermedades

Más Videos Relacionados

Rapid In Vivo Fixation and Isolation of Translational Complexes from Eukaryotic Cells
14:29

Rapid In Vivo Fixation and Isolation of Translational Complexes from Eukaryotic Cells

Published on: December 25, 2021

4.6K
Analysis of Cap-binding Proteins in Human Cells Exposed to Physiological Oxygen Conditions
10:40

Analysis of Cap-binding Proteins in Human Cells Exposed to Physiological Oxygen Conditions

Published on: December 28, 2016

8.2K

Videos de Experimentos Relacionados

Last Updated: Jan 8, 2026

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells
08:47

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells

Published on: May 1, 2020

3.3K
Rapid In Vivo Fixation and Isolation of Translational Complexes from Eukaryotic Cells
14:29

Rapid In Vivo Fixation and Isolation of Translational Complexes from Eukaryotic Cells

Published on: December 25, 2021

4.6K
Analysis of Cap-binding Proteins in Human Cells Exposed to Physiological Oxygen Conditions
10:40

Analysis of Cap-binding Proteins in Human Cells Exposed to Physiological Oxygen Conditions

Published on: December 28, 2016

8.2K

Área de la Ciencia:

  • Biología Molecular; Biología Celular; Bioquímica

Sus antecedentes:

  • La iniciación de la traducción es un proceso altamente regulado.; El complejo del factor de iniciación eucariota 4F (eIF4F) juega un papel clave en esta regulación.; El eIF4F consta de las subunidades eIF4E, eIF4A y eIF4G.

Objetivo del estudio:

  • Revisar las funciones del eIF4F en la traducción.; Discutir sus funciones reguladoras en diversas condiciones celulares.; Destacar las preguntas sin resolver sobre sus contribuciones mecanicistas.

Principales métodos:

  • Revisión de la literatura de estudios existentes sobre eIF4F.; Análisis de la estructura y función del eIF4F.; Examen de la participación del eIF4F en la regulación celular.

Principales resultados:

  • El eIF4F es central para la iniciación de la traducción, que está estrictamente regulada.; La desregulación del eIF4F está relacionada con numerosas anomalías fisiológicas.; La investigación reciente posiciona al eIF4F como un regulador celular global.

Conclusiones:

  • El eIF4F es fundamental para la función celular y el control traslacional.; Se necesita más investigación para comprender completamente las funciones mecanicistas del eIF4F.; La función del eIF4F se extiende más allá de la iniciación de la traducción, impactando la regulación celular global.