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Crioprotectores y dinámica del agua de la zona de exclusión

Alexis Theodorou¹, Karel Pomeisl¹, Jan Pokorný¹

¹FZU - Institute of Physics of the Czech Academy of Sciences, Na Slovance 2 182 00, Prague 8, Czech Republic.

|December 17, 2025

Resumen

Este estudio revela cómo los crioprotectores como los polietilenglicoles (PEG) y la trehalosa impactan la Zona de Exclusión (ZE) cerca de membranas hidrofílicas. La trehalosa mostró una menor supresión de la ZE que el PEG, lo que sugiere un papel en la estabilización del agua interfacial para la criopreservación.

Área de la Ciencia:

Ciencia Interfacial
Biofísica
Ciencia de Materiales

Sus antecedentes:

Las membranas hidrofílicas son cruciales en los sistemas biológicos debido a sus superficies cargadas.
La Zona de Exclusión (ZE) es una región de agua interfacial con propiedades únicas.
Comprender el comportamiento de la ZE es clave para optimizar la criopreservación.

Objetivo del estudio:

Investigar la relación entre la ZE cerca de las membranas de Nafion y los cambios térmicos.
Examinar cómo los crioprotectores (PEG, trehalosa) afectan la ZE.
Vincular la hidratación interfacial con la eficacia crioprotectora.

Principales métodos:

Se estudió la ZE cerca de las membranas de Nafion a varias temperaturas.
Se evaluaron los efectos de los polietilenglicoles (PEG) y la trehalosa sobre la ZE.
Se utilizó el seguimiento fluorescente de crioprotectores marcados con dansil.

Principales resultados:

El PEG y la trehalosa afectaron diferencialmente el comportamiento de la ZE.
La trehalosa exhibió una supresión más leve del tamaño de la ZE en comparación con el PEG.
Demostró interacciones distintas a larga distancia del agua interfacial con el PEG y la trehalosa.

Conclusiones:

Primera evidencia que vincula la actividad de los crioprotectores con el comportamiento de la ZE.
Sugiere que la ZE juega un papel en la estabilización del agua interfacial durante el enfriamiento.
Implicaciones para la criopreservación y los procesos asociados a las membranas.

Palabras clave:

Crioprotectores Marcador Dansil Agua de zona de exclusión Nafion Polietilenglicol Trehalosa

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