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The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...
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Video Experimental Relacionado

Updated: Jan 7, 2026

An Intravital Microscopy-Based Approach to Assess Intestinal Permeability and Epithelial Cell Shedding Performance
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ST3Gal1 modula la función de barrera intestinal e impacta la colitis ulcerosa humana

Yin Tian1, Yun Liu2, Yangyang Shang2

  • 1Department of Gastroenterology, The People's Hospital of Yubei District of Chongqing City, Chongqing 401120, P.R. China.

Molecular medicine reports
|December 19, 2025
PubMed
Resumen

La proteína ST3Gal1 regula la función de barrera intestinal. La supresión de ST3Gal1 mejora la integridad de la barrera, mientras que la sobreexpresión la deteriora, impactando la patogénesis de la enfermedad inflamatoria intestinal.

Palabras clave:
ST3Gal1barrerainflamaciónsialilaciónmodelo de triple cultivo

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Área de la Ciencia:

  • Gastroenterología
  • Biología Celular
  • Bioquímica

Sus antecedentes:

  • La patogénesis de la enfermedad inflamatoria intestinal (EII) implica la disfunción de la barrera de la mucosa intestinal.
  • La sialilación de proteínas es crucial para mantener la integridad de la barrera intestinal.
  • El papel de la α2,3-sialilación mediada por ST3Gal1 en la EII aún no se ha dilucidado por completo.

Objetivo del estudio:

  • Investigar el impacto de la α2,3-sialilación catalizada por ST3Gal1 en la función de barrera intestinal.
  • Explorar el papel de ST3Gal1 en la patogénesis de la colitis ulcerosa (CU).

Principales métodos:

  • Se estableció un modelo in vitro de triple cultivo que simula estados intestinales humanos sanos e inflamados.
  • Se utilizaron vectores adenovirales y lentivirales para la reducción y sobreexpresión de ST3Gal1 en células epiteliales intestinales (CEI).
  • Se evaluó la función de barrera intestinal utilizando la resistencia eléctrica transepitelial y ensayos de permeabilidad con FITC-dextrano.

Principales resultados:

  • La expresión de ST3Gal1 se correlacionó con la inflamación intestinal en pacientes con CU y un modelo de ratón con colitis.
  • La supresión de la expresión de ST3Gal1 mejoró la función de barrera intestinal; la sobreexpresión la deterioró.
  • ST3Gal1 moduló la expresión de MUC2, TFF3, CDX2, p-STAT3, IL-1β, IL-6 e IL-8, influyendo en la integridad de la barrera y la inflamación.

Conclusiones:

  • ST3Gal1 juega un papel importante en la regulación de la función de barrera intestinal.
  • La α2,3-sialilación catalizada por ST3Gal1 impacta la patogénesis de la CU al afectar las proteínas asociadas a la barrera y los mediadores inflamatorios.
  • La focalización de ST3Gal1 puede ofrecer potencial terapéutico para las enfermedades inflamatorias intestinales.