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Protocolo para la medición de la captación de glucosa in vivo, ex vivo e in vitro en adipocitos marrones

Chul-Hong Park¹, Ji Suk Chang¹

¹Laboratory of Gene Regulation and Metabolism, Pennington Biomedical Research Center, Baton Rouge, LA 70808, USA.

|December 20, 2025

Abstract:

Here, we present a protocol for assessing in vivo glucose uptake in brown adipose tissue (BAT) from mice using 2-deoxy-D-[1,2=-³H]glucose. We describe steps for reagent and animal preparation, tracer administration, and tissue processing to quantify radiolabeled glucose uptake and its conversion to glucose-6-phosphate. We then detail procedures for conducting glucose uptake assays in freshly isolated BAT and cultured brown adipocytes, enabling assessment under a range of experimental conditions. For complete details on the use and execution of this protocol, please refer to Park et al.¹.

Palabras clave:

Ciencias de la Salud Metabolismo ensayos basados en células

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