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Updated: Jan 8, 2026
Rapid Lipid Droplet Isolation Protocol Using a Well-established Organelle Isolation Kit
Published on: April 19, 2019
Protocolo para el aislamiento de gotitas lipídicas y mitocondrias de células de leucemia mieloide aguda para análisis
Ran Zhao1, Liang Zhang1, Shuxin Dong2
1Section of Molecular Hematology and Therapy, Department of Leukemia, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
Abstract:
Lipid droplets (LDs) are dynamic organelles critical for lipid and energy homeostasis. LD dysregulation is implicated in various diseases, including acute myeloid leukemia (AML), which exhibits aberrant lipid metabolism and chemoresistance. Here, we describe an effective protocol for the co-isolation of LDs and mitochondria from lipid-deficient cell types, e.g., AML cells. We detail procedures for the isolation, quality verification, and results from mass spectrometry-based lipidomic analysis. This protocol enables the functional study of organellar lipidomic regulation in AML.
