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Updated: Jan 8, 2026

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Protocolo para la extracción de regiones intergénicas de genomas anotados utilizando TIGRE

Breno Dupin¹, Matheus Sanita Lima², Alexandre Rossi Paschoal³

¹Department of Computer Science, Federal University of Technology-Parana, Cornelio Procopio, Paraná 86300-000, Brazil.

|December 20, 2025

Abstract:

Here, we present a protocol for extracting intergenic regions from annotated genomes using TIGRE (Tool for Intergenic Region Extraction). This protocol describes steps for installing and running TIGRE locally. Via TIGRE, the user can prepare the input data ("clean" command), retrieve the intergenic sequences ("extract" command), obtain the respective intergenic nucleotide sequences ("getfasta" command), and merge different GFF3 files ("combine" command). TIGRE capabilities are demonstrated on the retrieval of intergenic sequences from 1,207 land plant mitochondrial genomes.

Palabras clave:

bioinformática ciencias de la computación biología evolutiva genómica

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