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Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response

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Análisis Transcriptómico Global Identifica Programas de Expresión Génica Regulados por el Factor α en Levadura

Soojin Yeom^1,2,3,4, Jung-Shin Lee^1,2

¹Department of Molecular Bioscience, College of Biomedical Science, Kangwon National University, Chuncheon 24341, Republic of Korea.

Journal of microbiology and biotechnology

|December 21, 2025

Resumen

Este estudio revela cambios en la expresión génica a nivel genómico en células de levadura que responden a la feromona alfa. Identifica cientos de genes involucrados en el apareamiento, la remodelación de la pared celular y la regulación del ciclo celular.

Palabras clave:

Saccharomyces cerevisiae arresto del ciclo celular remodelación de la cromatina señalización de feromonas factor α

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Área de la Ciencia:

Biología Molecular
Genética de Levaduras
Señalización Celular

Sus antecedentes:

La feromona alfa de levadura induce una cascada de señalización en células haploides "a".
Las respuestas fisiológicas conocidas incluyen la detención del ciclo celular G1, el crecimiento polarizado (formación de shmoo) y la fusión celular.
Anteriormente, faltaba un análisis transcripcional completo a nivel genómico.

Objetivo del estudio:

Realizar un análisis a nivel genómico de los cambios transcripcionales en células de levadura de tipo de apareamiento "a" tratadas con alfa-factor.
Identificar genes expresados diferencialmente (DEGs) en respuesta a la señalización de feromonas.
Proporcionar un recurso para comprender las respuestas de apareamiento en levaduras.

Principales métodos:

Se empleó la secuenciación de ARN (RNA-seq) para perfilar la expresión génica.
Se trataron células de levadura de tipo de apareamiento "a" con alfa-factor.
Se utilizó el análisis de ontología génica (GO) para interpretar el enriquecimiento funcional de los DEGs.

Principales resultados:

Identificó 957 genes expresados diferencialmente (DEGs): 448 regulados al alza y 509 regulados a la baja.
Los genes regulados al alza se enriquecen en la señalización de feromonas, la biogénesis de la pared celular y la regulación de la forma celular.
Los genes regulados a la baja se asocian con la progresión del ciclo celular, la remodelación de la cromatina y la organización de nucleosomas.

Conclusiones:

El estudio proporciona un valioso conjunto de datos de genes de levadura que responden a feromonas.
Se identificaron nuevos genes candidatos involucrados en las respuestas de apareamiento.
Los hallazgos ofrecen información sobre la coordinación de la regulación transcripcional y la dinámica de la cromatina durante la señalización de feromonas en levaduras.