Parche de Microagujas Multifacético: Una Solución Integral para Combatir Infecciones de Heridas de Múltiples Tipos

Área de la Ciencia:

• Biomaterials Science; Regenerative Medicine; Wound Healing

Sus antecedentes:

• Bacterial-infected wounds present a global health challenge, complicated by polymicrobial infections and altered microenvironments.; Current treatments struggle with real-time monitoring and targeted intervention in complex wound scenarios.

Objetivo del estudio:

• To develop a microenvironment-responsive microneedle (MN) patch for dynamic diagnosis and graded intervention in bacterial-infected wounds.; To integrate a visual pH warning system with enzyme-triggered exosome delivery and multi-therapeutic payloads.

Principales métodos:

• Fabrication of an MN patch incorporating a bromothymol blue pH indicator, gelatin methacryloyl/exosome matrix for enzyme-triggered release, and halloysite nanotubes, antimicrobial peptides, and salvianolic acid B.; In vitro assessment of antibacterial activity against MRSA/IPRPA, biofilm inhibition, and disruption.; In vivo evaluation in acute and chronic infected wound models, including diabetic ulcers, assessing healing rates, inflammation markers, and angiogenesis.

Principales resultados:

• Exceeded 95% bacterial clearance and 90% biofilm inhibition/disruption in vitro.; MNs provided visual pH changes within 8 hours in acute and chronic wounds.; Accelerated acute wound healing within 10 days and improved diabetic ulcer healing by 9.20% compared to controls, with significant inflammation reduction and angiogenesis promotion.

Conclusiones:

• The developed MN patch effectively integrates real-time diagnosis (pH monitoring) with graded therapeutic intervention.; This platform demonstrates significant potential for advancing the management of complex and chronic infected wounds, including diabetic ulcers.; The dynamic warning-graded intervention strategy offers a promising approach for personalized and efficient wound care.