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Updated: May 6, 2026

Identification of Metabolically Active Bacteria in the Gut of the Generalist Spodoptera littoralis via DNA Stable Isotope Probing Using 13C-Glucose
Published on: November 13, 2013
Composición de la microbiota intestinal de larvas de Trichoptera en grupos funcionales de alimentación: un estudio de
Gabriela Jijón1, Connor Hough1, Danyon Gedris1
1Department of Plant and Wildlife Sciences, Brigham Young University, Provo, UT 84602, USA Brigham Young University Provo United States of America.
Abstract:
Diet is known to be a major factor in the gut microbiome of many groups of insects. Larvae from the insect order Trichoptera have varied feeding behaviors, encompassing all functional feeding groups, making them an excellent group for studying the links between diet and gut microbe community variation. However, no previous study has examined these links in caddisflies. Here, we characterize the gut microbiome composition of four caddisfly genera belonging to four different functional feeding groups over two sampling periods using 16S metabarcoding. We found that the sampling date had the strongest influence on gut microbiome variation. Host functional feeding groups and phylogeny also influenced gut community composition; however, improved sampling is necessary to confirm this relationship. Our preliminary results point to interesting differences among larvae from different feeding groups and suggest future areas for research, including performing species-level identification of the caddisfly larvae, using more taxa within and between functional feeding groups, using temporal and larval stage-matched replicates, assessing the degree of microbiome residency in caddisfly guts, and performing deeper sequencing.
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