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Secuencias completas del genoma de siete fagos del clúster DE1 aislados utilizando Gordonia terrae
Azzam Almutairi1, Gabriella Marsala2, Natalie Mershon3
1Department of Biochemistry, The Ohio State University, Columbus, Ohio, United States.
microPublication biology
|December 22, 2025
Resumen
Se aislaron siete nuevos bacteriófagos del suelo utilizando Gordonia terrae. Estos fagos, asignados al clúster de actinobacteriófagos DE1, presentan tamaños de genoma de entre 55.593 pb y 59.501 pb.
Área de la Ciencia:
- Microbiología
- Genómica
- Virología
Sus antecedentes:
- Los bacteriófagos son virus que infectan bacterias y desempeñan roles cruciales en los ecosistemas microbianos.
- Los actinobacteriófagos son un grupo diverso de fagos que infectan actinobacterias, incluyendo géneros importantes como *Gordonia*.
- Los entornos del suelo albergan una gran diversidad de bacteriófagos con aplicaciones biotecnológicas potenciales.
Objetivo del estudio:
- Aislar y caracterizar nuevos bacteriófagos de muestras de suelo.
- Determinar las características genómicas de los fagos aislados.
- Clasificar los fagos según su relación genética.
Principales métodos:
- Aislamiento de bacteriófagos del suelo utilizando la bacteria huésped *Gordonia terrae* 3612.
- Secuenciación del genoma completo de los fagos aislados.
- Análisis bioinformático del tamaño del genoma, contenido génico y relaciones filogenéticas.
Principales resultados:
- Se aislaron con éxito siete bacteriófagos, denominados Barsten, Kamashten, Kwobi, LilHam, SchottB, StorminNorm y Thing3.
- Los genomas de estos fagos varían de 55.593 a 59.501 pares de bases.
- Cada genoma de fago contiene un promedio de 85 genes que codifican proteínas.
- El análisis filogenético basado en la similitud del contenido génico asignó los siete fagos al clúster de actinobacteriófagos DE1.
Conclusiones:
- El estudio identificó y caracterizó con éxito siete nuevos bacteriófagos que infectan *Gordonia terrae*.
- Estos fagos representan nuevos miembros del clúster de actinobacteriófagos DE1, ampliando nuestra comprensión de la diversidad de fagos.
- Los datos genómicos proporcionan una base para una mayor investigación sobre la biología y las aplicaciones potenciales de estos fagos.
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