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Updated: Jul 25, 2026
A High Content Imaging Assay for Identification of Botulinum Neurotoxin Inhibitors
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“Centinela de Nanocinta”: un aptasensor de superficie celular para la obtención de imágenes en tiempo real de la
Meirong Cui1,2,3, Chenxu Hu1,2,3, Yiyu Cheng1,2,3
1State Key Laboratory for Flexible Electronics (LoFE), Nanjing University of Posts and Telecommunications, Nanjing, China. iamjchao@njupt.edu.cn.
Abstract:
We report a cell-surface-anchored "Nanoribbon Sentinel", an aptasensor overcoming endocytosis for real-time, high-resolution imaging of ATP on live astrocytes. This design uniquely couples a non-internalizing scaffold with a fast-kinetic "signal-on" aptamer, enabling high temporal resolution. The sensor successfully captured the transient, localized release of ATP from mechanically stimulated cells, offering a powerful new tool to study neurochemical signaling.
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