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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Updated: Jul 1, 2026

Isolation of Labile Multi-protein Complexes by in vivo Controlled Cellular Cross-Linking and Immuno-magnetic Affinity Chromatography

Isolation of Labile Multi-protein Complexes by in vivo Controlled Cellular Cross-Linking and Immuno-magnetic Affinity Chromatography

Published on: March 9, 2010

Cross-linkers multitargeting clickables based on SuFEx for in vivo cross-linking mass spectrometry

Yanhong Hao¹, Moran Chen¹, Xiao Huang¹

¹The Institute for Advanced Studies, Renmin Hospital of Wuhan University, Wuhan University, Wuhan, Hubei 430072, China.

Analytical chemistry

|December 23, 2025

Resumen

Los investigadores desarrollaron nuevos cross-linkers trifuncionales (SPSF) para la espectrometría de masas de entrecruzamiento in vivo (XL-MS). Esta técnica mejora el análisis de la estructura de las proteínas al aumentar la cobertura de aminoácidos e identificar nuevos sitios de entrecruzamiento, incluidos residuos asociados a la fosforilación.

Palabras clave:

espectrometría de masas de entrecruzamiento química de clics proteómica estructura de proteínas interacciones de proteínas SuFEx SPSF cross-linker in vivo trifuncional

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