Video Experimental Relacionado

Updated: Jul 1, 2026

Isolation of Labile Multi-protein Complexes by in vivo Controlled Cellular Cross-Linking and Immuno-magnetic Affinity Chromatography
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Isolation of Labile Multi-protein Complexes by in vivo Controlled Cellular Cross-Linking and Immuno-magnetic Affinity Chromatography

Published on: March 9, 2010

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Cross-linkers multitargeting clickables based on SuFEx for in vivo cross-linking mass spectrometry

Yanhong Hao1, Moran Chen1, Xiao Huang1

  • 1The Institute for Advanced Studies, Renmin Hospital of Wuhan University, Wuhan University, Wuhan, Hubei 430072, China.

Analytical chemistry
|December 23, 2025
PubMed
Resumen

Los investigadores desarrollaron nuevos cross-linkers trifuncionales (SPSF) para la espectrometría de masas de entrecruzamiento in vivo (XL-MS). Esta técnica mejora el análisis de la estructura de las proteínas al aumentar la cobertura de aminoácidos e identificar nuevos sitios de entrecruzamiento, incluidos residuos asociados a la fosforilación.

Palabras clave:
espectrometría de masas de entrecruzamientoquímica de clicsproteómicaestructura de proteínasinteracciones de proteínasSuFExSPSFcross-linkerin vivotrifuncional

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