Jove
Visualize
Contáctanos

Videos de Conceptos Relacionados

Infection01:20

Infection

11.6K
When a pathogen enters the body and reproduces, it can cause an infection, damage body cells, and cause illness symptoms that eventually lead to disease. Therefore, its prevention requires breaking the chain of infection.
The chain begins with pathogens: bacteria, viruses, fungi, prions, or parasites such as protozoa helminths. These can be present on the skin as transient or resident flora, or they can be acquired from the environment. Identifying and treating the type of infection and...
11.6K
Urinary Tract Infection II: Pathophysiology01:25

Urinary Tract Infection II: Pathophysiology

524
The pathophysiology of urinary tract infections (UTIs) encompasses several progressive stages, beginning with bacterial colonization and culminating in potential systemic complications if untreated. UTIs are primarily initiated by bacteria, such as Escherichia coli, which often originate from the gastrointestinal tract and migrate to the urinary system through the periurethral area. This migration can occur via several routes, including improper hygiene practices, sexual activity, or...
524
Cystic Fibrosis: Pathogenesis01:23

Cystic Fibrosis: Pathogenesis

676
Cystic fibrosis (CF), an autosomal recessive disorder, significantly affects the function of exocrine glands. This genetically inherited disease is characterized by the production of thick and sticky mucus, which can severely affect various organs and systems in the body.
CF is primarily caused by a genetic mutation in a chromosome 7 gene coding for the cystic fibrosis transmembrane conductance regulator (CFTR) protein. The most common gene mutation leading to CF is the ΔF508 mutation,...
676
Pneumonia II: Pathophysiology01:29

Pneumonia II: Pathophysiology

2.5K
The pathophysiology of pneumonia involves the following steps:
2.5K
Stages of Infection01:26

Stages of Infection

64.7K
Stages of infection describe what happens to a susceptible host once a pathogen invades the human body. The stages of infection are incubation, prodromal, illness, stage of decline, and convalescence. The incubation stage is the period from exposure to a pathogen until symptoms start. The infected person is unaware of impending illness as the pathogens grow and multiply within the body. The duration may vary depending on the type of infection. The incubation period of measles averages ten to...
64.7K
Defense Against Bacterial Pathogens01:31

Defense Against Bacterial Pathogens

2.6K
The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
2.6K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Therapeutic targeting of fibrin-microglia interactions ameliorates Alzheimer's disease-related hyperexcitability and brain network dysfunction.

bioRxiv : the preprint server for biology·2026
Same author

Resilience to neuronal hyperactivity and restoration of the neuroimmune interactome by blocking fibrin-induced microglia activation in Alzheimers disease.

bioRxiv : the preprint server for biology·2026
Same author

Parvalbumin Neuron-Targeted Loss of Alzheimer's Disease Risk Gene <i>BIN1</i> Is Insufficient to Drive Cognitive or Network Excitability Changes.

eNeuro·2026
Same author

cGAS-mediated IFN-I signaling contributes to disease progression in drug-refractory epilepsy.

bioRxiv : the preprint server for biology·2026
Same author

Sleep-Wake Transitions Are Impaired in the <i>App</i> <sup>NL-G-F</sup> Mouse Model of Early Onset Alzheimer's Disease.

bioRxiv : the preprint server for biology·2026
Same author

APOE4-Aβ synergy drives brain network dysfunction and neuronal lysosomal-ER proteostasis dysregulation a preclinical Alzheimer's disease model.

bioRxiv : the preprint server for biology·2025
Same journal

Breaking barriers: Enhancing access to dementia clinical trials in the United Kingdom-Insights from the Scientific Advisory Board of the Dame Barbara Windsor Dementia Goals Programme.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2026
Same journal

Unveiling the procoagulant state in Alzheimer's disease: A novel PET imaging strategy.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2026
Same journal

Estimated labor market outcomes of people progressing from preclinical to early-stage Alzheimer's disease in the United States.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2026
Same journal

Amyloid exacerbates tau and alpha-synuclein pathologies, behavioral impairments, and neuroinflammation in a mixed dementia model.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2026
Same journal

Multimorbidity burden and patterns associated with DeepBrainNet-derived brain-age gap in dementia-free older adults: A community-based study.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2026
Same journal

Reply to "Shifting the emphasis of brain health literacy from individuals to systems to reduce inequalities".

Alzheimer's & dementia : the journal of the Alzheimer's Association·2026
Ver todos los artículos relacionados
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Video Experimental Relacionado

Updated: Jan 8, 2026

Mouse Footpad Inoculation Model to Study Viral-Induced Neuroinflammatory Responses
09:07

Mouse Footpad Inoculation Model to Study Viral-Induced Neuroinflammatory Responses

Published on: June 14, 2020

11.5K

Ciencia básica y patogénesis

Jia Shin1, Erica Brady1, Stephanie R Miller1

  • 1Gladstone Institute of Neurological Disease, University of California San Francisco, San Francisco, CA, USA.

Alzheimer's & dementia : the journal of the Alzheimer's Association
|December 23, 2025
PubMed
Resumen
Este resumen es generado por máquina.

La combinación de ApoE4 humanizado con mutaciones AppNL-F en ratones provocó hipoactividad e neuroinflamación. La reducción de las placas de Aβ en estos ratones resalta las diferencias entre especies, enfatizando la necesidad de modelos humanizados en la enfermedad de Alzheimer.

Palabras clave:
Enfermedad de AlzheimerApoE4AppNL-Fratonesneuroinflamaciónplacas de Aβmodeladomodelos humanizados

Más Videos Relacionados

A Precise Pathogen Delivery and Recovery System for Murine Models of Secondary Bacterial Pneumonia
13:45

A Precise Pathogen Delivery and Recovery System for Murine Models of Secondary Bacterial Pneumonia

Published on: September 21, 2019

6.0K
Using a Bacterial Pathogen to Probe for Cellular and Organismic-level Host Responses
08:38

Using a Bacterial Pathogen to Probe for Cellular and Organismic-level Host Responses

Published on: February 22, 2019

6.3K

Videos de Experimentos Relacionados

Last Updated: Jan 8, 2026

Mouse Footpad Inoculation Model to Study Viral-Induced Neuroinflammatory Responses
09:07

Mouse Footpad Inoculation Model to Study Viral-Induced Neuroinflammatory Responses

Published on: June 14, 2020

11.5K
A Precise Pathogen Delivery and Recovery System for Murine Models of Secondary Bacterial Pneumonia
13:45

A Precise Pathogen Delivery and Recovery System for Murine Models of Secondary Bacterial Pneumonia

Published on: September 21, 2019

6.0K
Using a Bacterial Pathogen to Probe for Cellular and Organismic-level Host Responses
08:38

Using a Bacterial Pathogen to Probe for Cellular and Organismic-level Host Responses

Published on: February 22, 2019

6.3K

Área de la Ciencia:

  • Neurociencia
  • Genética
  • Biología Molecular

Sus antecedentes:

  • La apolipoproteína E4 (ApoE4) humanizada es un factor de riesgo genético importante para la enfermedad de Alzheimer (EA) de aparición tardía.
  • La ApoE4 se asocia con disfunción sináptica, neuroinflamación y alteración de la depuración de beta-amiloide (Aβ).
  • El modelo de ratón de inserción genética AppNL-F replica la fisiopatología humana de Aβ manteniendo la regulación génica nativa.

Objetivo del estudio:

  • Investigar los efectos combinados de las mutaciones AppNL-F y ApoE4 humanizada en la patogénesis de la EA.
  • Abordar los desafíos en el modelado de la biología humana de ApoE en ratones.
  • Avanzar en las perspectivas traslacionales sobre la EA.

Principales métodos:

  • Se generaron cuatro genotipos: ApoE4/AppNL-F (E4NLF), ApoE4 (E4), AppNL-F (NLF) y tipo salvaje (WT).
  • Se utilizó la prueba de comportamiento en campo abierto con análisis de aprendizaje automático.
  • Se cuantificaron las placas de Aβ y la reactividad microglial mediante inmunohistoquímica.
  • Se midieron los niveles de Aβ42/40 soluble mediante ELISA y se identificaron genes expresados diferencialmente (DEGs) mediante snRNA-seq.

Principales resultados:

  • Los ratones E4NLF exhibieron hipoactividad, habituación alterada y aumento de movimientos lentos.
  • Los ratones E4NLF mostraron significativamente menos placas de Aβ hipocampales en comparación con los ratones NLF.
  • La microglía mostró activación cerca de las placas en los genotipos NLF y E4NLF.
  • La reducción de la expresión de Ttr en astrocitos y microglía E4NLF sugirió una alteración en la depuración de Aβ.
  • Las neuronas excitatorias mostraron el mayor número de DEGs en ratones E4NLF.

Conclusiones:

  • La combinación de las mutaciones ApoE4 y AppNL-F induce fenotipos distintos relacionados con la EA, incluida la neuroinflamación y la alteración de la función glial.
  • La reducción de las placas de Aβ en ratones E4NLF subraya las diferencias entre especies en la depuración de Aβ y la función de la isoforma de ApoE.
  • Las limitaciones del ApoE de ratón en el modelado de la biología humana de ApoE exigen modelos humanizados para estudiar el papel de ApoE en la EA.