Ciencia básica y patogénesis

Alterations in circadian rhythms are widely observed in Alzheimer's disease (AD) and other neurodegenerative disorders. However, the significance of ex-vivo cellular circadian periods and their deviation from the 24-hour cycle, measured in patient-derived fibroblasts, remains largely unexplored.

We analyzed 135 older adults with cognitive complaints from the BICWALZS cohort. Cellular circadian periods and their deviation from 24 hours were measured in patient-derived dermal fibroblasts using a lentiviral vector reporting Bmal1 transcription via real-time luciferase assays. Biomarkers reflecting revised ATN(IV) criteria for AD, including plasma biomarkers and neuroimaging, were assessed. Exploratory correlation analyses and generalized linear models adjusted for covariates identified associations. Voxel-based morphometry explored regional grey matter density, and survival analysis evaluated longitudinal clinical progression.

The cellular circadian period was significantly associated with tau proteinopathy (plasma pTau-217, p = 0.012, small effect size), neural injury and degeneration (plasma NfL, p <0.001, large effect size; MRI Schelten's scale, p = 0.033, small effect size), and inflammation (plasma GFAP, p <0.001, medium effect size). Longer periods correlated with reduced grey matter density in AD-relevant regions, including the left and right amygdala (p = 0.027 and p = 0.040, respectively) and the left parahippocampal gyrus (p = 0.042). Deviation from 24 hours was strongly associated with age (p = 0.02, FDR-p=0.06), neurodegeneration (MRI Schelten's scale, p = 0.004), cognitive decline (MMSE, p = 0.016), and memory and language impairments (all p <0.05). Voxel-based morphometry revealed broader associations for period deviation with grey matter density in aging- and neurodegeneration-related regions, including the hippocampus and superior/middle temporal poles. Survival analysis showed that longer cellular circadian period were associated with faster clinical progression (HR=3.59, p = 0.01).

Cellular circadian metrics, particularly period deviation, reflect aging-related neurodegenerative changes and cognitive impairments, while longer periods are linked to AD-specific pathology and faster clinical progression. These findings highlight their potential as biomarkers for AD and aging-related neurodegeneration, warranting further investigation.