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Updated: Jan 7, 2026

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

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Protocolo para la recuperación y secuenciación profunda de pools cortos de ADNss a partir de gotitas de coacervado

Anna-Lena Holtmannspötter¹, Corbin Machatzke², Job Boekhoven¹

¹Department of Bioscience, School of Natural Sciences, Technical University of Munich, Lichtenbergstrasse 4, 85748 Garching, Germany.

|December 25, 2025

Abstract:

Complex coacervate droplets are synthetic cell models that sequester nucleic acids. Here, we present a protocol for the recovery and deep sequencing of single-stranded DNA (ssDNA) from metabolically active coacervate droplets. We describe time-resolved harvesting, ssDNA recovery, and sequence-independent library preparation for next generation sequencing (NGS), along with an analysis pipeline to assess enrichment dynamics and sequence distributions. The protocol is adaptable to diverse droplet systems. For complete details on the use and execution of this protocol, please refer to Machatzke et al.¹.

Palabras clave:

química análisis de secuencias secuenciación

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