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Updated: Jan 7, 2026

In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy

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Published on: August 6, 2014

Protocolo para corte 3D y fenotipado celular profundo mediante imagen de hoja de luz y multiplexación espacial 2D

Kevin Bigott¹, Victoria H Schoppel¹, Manuel Martinez-Osuna¹

¹Miltenyi Biotec B.V. & Co. KG, 51429 Bergisch Gladbach, Germany.

|December 28, 2025

Abstract:

Spatial analysis of cells and their microenvironment within tissues enhances our understanding of biological processes. Ideally, a broad range of biomolecules should be analyzed in large 3D tissue specimens at subcellular resolution. Here, we present a protocol to identify and extract target sections from previously cleared tissues. We describe steps for combining 3D light sheet imaging and subsequent 3D-guided deep cell phenotyping via multi-cyclic 2D microscopy.

Palabras clave:

Cáncer Microscopía Neurociencia

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