Video Experimental Relacionado
Updated: Jan 7, 2026
Establishing a Mouse Contusion Spinal Cord Injury Model Based on a Minimally Invasive Technique
Published on: September 7, 2022
Protocol para desarrollar un modelo murino de lesión medular espinal (SCI) por contusión-compresión grave para
Sissi Dolci1, Marzia Di Chio1, Giuseppe Busetto2
1Laboratory of Pharmacology, Department of Diagnostic and Public Health, University of Verona, 37134 Verona, Italy.
Abstract:
Complete spinal cord injury (SCI) leads to irreversible neurological damage due to failed neural repair, with no effective therapies currently available. Here, we present a protocol to induce severe contusive-compressive SCI at thoracic T11 level in mouse using the NYU-MASCIS II impactor. We describe steps for performing laminectomy, inducing the injury, and validating it through functional and histological analysis. This protocol replicates key aspects of human secondary injury, making it valuable for preclinical testing of SCI therapies.
