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Nueva endolisina del profago específico de Streptococcus iniae inhibe selectivamente bacterias diana
JoonBeom Moon1, Hanbeen Kim2, Suryang Kwak3
1Department of Animal Science, Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463 Republic of Korea.
Journal of microbiology and biotechnology
|December 30, 2025
Resumen
Una nueva endolisina, SinLys1930, se dirige y elimina eficazmente Streptococcus iniae, un patógeno clave en la acuicultura. Este descubrimiento ofrece una prometedora alternativa a los antibióticos para prevenir la estreptococosis y reducir las pérdidas económicas.
Área de la Ciencia:
- Microbiología
- Biotecnología
- Salud Acuícola
Sus antecedentes:
- Streptococcus iniae causa pérdidas económicas significativas en la acuicultura debido a la estreptococosis.
- La resistencia a los antibióticos exige el desarrollo de tratamientos alternativos.
- Las endolisinas derivadas de bacteriófagos ofrecen una actividad lítica específica contra patógenos bacterianos.
Objetivo del estudio:
- Desarrollar y caracterizar una nueva endolisina, SinLys1930, con actividad específica contra Streptococcus iniae.
- Evaluar la actividad lítica y las condiciones óptimas para SinLys1930.
- Evaluar el potencial de SinLys1930 como alternativa a los antibióticos en la acuicultura.
Principales métodos:
- Predicción bioinformática de las características moleculares y estructurales de SinLys1930.
- Evaluación de la actividad lítica contra S. iniae en diversas condiciones (pH, temperatura, salinidad, iones metálicos).
- Prueba de la eficacia de SinLys1930 en agua de mar esterilizada.
Principales resultados:
- SinLys1930 posee dos dominios enzimáticamente activos y dos dominios de unión a la pared celular.
- Se observó una actividad lítica óptima a pH 6,0-6,5 y 16-37 °C.
- SinLys1930 demostró una reducción significativa (aproximadamente 60%) en la densidad óptica de S. iniae en agua de mar en 1 hora.
Conclusiones:
- SinLys1930 exhibe una potente actividad lítica contra Streptococcus iniae.
- La endolisina muestra estabilidad en diversas condiciones ambientales relevantes para la acuicultura.
- SinLys1930 presenta una alternativa viable a los antibióticos para el manejo de la estreptococosis en la acuicultura.
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