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Formación de Redes de Enlace de Coordinación de Platino(II) Inducida en Coacervados de ADN de Cadena Sencilla

Ryu Tashiro¹, Takuma Yamada², Serika Yano¹

¹Faculty of Pharmaceutical Sciences, Suzuka University of Medical Science, Suzuka, Mie 513-8670, Japan.

Inorganic chemistry

|December 30, 2025

Abstract:

Coacervates are dense aqueous phases that form by liquid-liquid phase separation. Seven Pt(II) complexes with different charges and nucleotide reactivities were examined for their ability to induce coacervate formation in a 21-mer single-stranded DNA (ssDNA). Only AMPZ ([cis-{Pt(NH₃)₂}₂(μ-pyrazolato)(μ-OH)](NO₃)₂), a cationic dinuclear Pt(II) complex, efficiently induced coacervate formation in ssDNA containing only thymine (T21-DNA). AMPZ has very low reactivity with thymine but relatively high reactivity with guanine, and when three of the thymines in T21-DNA were substituted with a guanine to produce T18-G3-DNA, the resulting coacervate was observed to undergo gelation via the formation of an extensive Pt-DNA coordination-bonded network. We then examined the construction of coacervates that comprise multiple phases by adding AMPZ to a mixture of two types of ssDNAs, a highly reactive T10-G11-DNA and a minimally reactive T21-DNA, and found that two distinct assembly states─a cell mimetic assembly and a DNA-encapsulating gel─could be formed.

Palabras clave:

ADN de cadena sencilla coacervados complejos de platino redes de coordinación gelación materiales biomiméticos

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