Visualización y evaluación precisa de la lesión pulmonar aguda sin diafonía mediante una sonda fluorescente de dos...

Acute lung injury (ALI) is an inflammatory lung disease with high morbidity and mortality, however effective treatments for it are still lacking. Thus, a real-time and accurate method for ALI visualization is urgently needed for research and therapeutic evaluation. In this work, we introduced a two-photon fluorescent probe LD-DCIE for dual-channel detection of viscosity in lipid droplets (LDs) and ONOO^-. LD-DCIE exhibited a near-infrared fluorescence at 660 nm in response to high viscosity, and provided a crosstalk-free blue fluorescence signal at 425 nm after reacting with ONOO^-. The probe displayed good two-photon properties and LDs targeting capability, and was successfully applied to reveal the changes of ONOO^- and viscosity in LPS-induced ALI model cells and mice through two crosstalk-free fluorescence channels. Moreover, the therapeutic effects of two drugs for ALI treatment, puerarin (Pue) and syringic acid (SA), were evaluated by LD-DCIE through the real-time visualization. Thus, probe LD-DCIE is a promising imaging tool to evaluate potential drugs and develop therapeutic strategies for ALI.