Video Experimental Relacionado

Updated: Jan 7, 2026

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

Published on: July 28, 2017

C-Nucleósidos Estabilizan el ARN al Reducir la Nucleofilicidad en 2'-OH

Dipanwita Banerjee¹, Lu Xiao¹, Pavitra S Thacker¹

¹Department of Chemistry, Stanford University, Stanford, California 94305, United States.

ACS central science

|December 31, 2025

Resumen

Los nucleótidos sustituidos con carbono, como la pseudouridina, ralentizan significativamente la degradación del ARN al reducir las tasas de escisión de las hebras. Este descubrimiento ofrece información sobre la estabilidad del ARN para procesos biológicos y aplicaciones terapéuticas.

Área de la Ciencia:

Bioquímica
Biología Molecular
Terapias de ARN

Sus antecedentes:

Los nucleótidos sustituidos con carbono son prevalentes en ARN biológicos y terapéuticos, incluida la pseudouridina y la N1-metilpseudouridina.
Se sabe que estas modificaciones mejoran la estabilidad del ARN, pero el mecanismo subyacente sigue sin estar claro.

Objetivo del estudio:

Investigar el mecanismo por el cual los nucleótidos sustituidos con carbono reducen la degradación del ARN.
Elucidar el impacto de la sustitución de carbono en la cinética de escisión del ARN y la nucleofilicidad.

Principales métodos:

Análisis cinético de la escisión espontánea y enzimática en enlaces individuales en ARN modificados sintéticamente.
Estudios sobre reacciones de acilación nucleofílica que involucran ARN modificados y alcoholes con pKa variable.

Principales resultados:

La sustitución de carbono disminuye significativamente las tasas de escisión de las hebras de ARN a través de vías espontáneas y enzimáticas.
Los efectos inductivos reducidos de la sustitución de carbono conducen a un pKa más alto y una menor nucleofilicidad en el ARN.

Conclusiones:

La sustitución de carbono en los nucleótidos mejora la estabilidad del ARN al reducir las tasas de escisión.
Los hallazgos proporcionan una comprensión mecanicista de las modificaciones del ARN relevantes para los transcriptomas nativos y las terapias basadas en ARN.

Palabras clave:

ARN estabilidad del ARN nucleótidos sustituidos con carbono pseudouridina terapias de ARN

Más Videos Relacionados

Regioselective O-Glycosylation of Nucleosides via the Temporary 2',3'-Diol Protection by a Boronic Ester for the Synthesis of Disaccharide Nucleosides

Regioselective O-Glycosylation of Nucleosides via the Temporary 2',3'-Diol Protection by a Boronic Ester for the Synthesis of Disaccharide Nucleosides

Published on: July 26, 2018

DNAzyme-dependent Analysis of rRNA 2’-O-Methylation

DNAzyme-dependent Analysis of rRNA 2’-O-Methylation

Published on: September 16, 2019

Videos de Conceptos Relacionados

RNA Stability

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...

RNA Structure

RNA Structure

The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...

RNA Structure

RNA Structure

Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...

Nucleic Acid Structure

Nucleic Acid Structure

The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...

Types of RNA

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...

Types of RNA

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...