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Revelando la diversidad oculta de parásitos: mitogenómica de lectura larga en Eimeriidae y Haemosporida (Eukaryota:
Peihang Hong1, Sijia Yu2, Hau-You Tzeng3
1Department of Veterinary Medicine, College of Veterinary Medicine, National Chung Hsing University, 145 Xing Da Rd., South District, Taichung City, Taiwan; Laboratory of Veterinary Parasitology, Nippon Veterinary and Life Science University, 1-7-1 Kyonancho, Musashino-shi, Tokyo, Japan.
International journal for parasitology
|December 31, 2025
Resumen
Este estudio introduce un nuevo método que utiliza Oxford Nanopore Technologies (ONT) para secuenciar genomas mitocondriales de parásitos apicomplejos. Revela una diversidad oculta de parásitos y mejora la comprensión de la evolución e infecciones de los apicomplejos.
Área de la Ciencia:
- Parasitología
- Genómica
- Biología Evolutiva
Sus antecedentes:
- Los parásitos apicomplejos muestran diversas estructuras de genoma mitocondrial.
- Las limitaciones técnicas han obstaculizado previamente la caracterización completa, especialmente en infecciones complejas.
- Comprender la diversidad de parásitos es crucial para los estudios de interacción huésped-parásito.
Objetivo del estudio:
- Desarrollar y validar un flujo de trabajo novedoso para la secuenciación completa de genomas mitocondriales de apicomplejos.
- Descubrir la diversidad críptica de parásitos utilizando tecnología de secuenciación avanzada.
- Realizar análisis genómicos comparativos y filogenómicos de Eimeriidae y Haemosporida.
Principales métodos:
- Flujo de trabajo integrado que combina la amplificación por PCR de un solo paso y la secuenciación de Oxford Nanopore Technologies (ONT).
- Ensamblaje de 29 genomas mitocondriales de alta calidad de Eimeriidae y Haemosporida.
- Análisis comparativo con secuenciación Sanger y reconstrucción filogenómica.
Principales resultados:
- Secuenciación exitosa de 29 mitogenomas, revelando infecciones mixtas/crípticas en 11 muestras.
- La secuenciación ONT demostró una resolución superior en comparación con la secuenciación Sanger.
- Los análisis filogenómicos proporcionaron información sobre las relaciones evolutivas y las presiones de selección de Eimeriidae y Haemosporida.
Conclusiones:
- El novedoso flujo de trabajo basado en ONT es sensible y eficaz para caracterizar genomas mitocondriales de apicomplejos.
- Este método mejora significativamente la detección de la verdadera diversidad de parásitos en infecciones complejas.
- Los hallazgos proporcionan un marco escalable para estudios de biodiversidad y evolución de apicomplejos.
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