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Super-resolution Fluorescence Microscopy01:37

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Atomic Force Microscopy01:08

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Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
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Video Experimental Relacionado

Updated: Jan 7, 2026

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
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La compensación de la detención mediada por ROS de la dinámica de la fenestración endotelial permite imágenes ópticas

Annika Kiel1, Marcin Luty2,3, Angela Kralemann-Köhler1

  • 1Department of General- and Visceral Surgery - Liver- and Tumor Biology, Medical Faculty OWL, Bielefeld University, Bielefeld 33615, Germany.

ACS applied materials & interfaces
|January 5, 2026
PubMed
Resumen

No abstract available in PubMed .

Palabras clave:
microscopía de fuerza atómica (AFM)las ventanasImágenes de células vivascélulas endoteliales sinusoidales del hígado (LSEC)especies reactivas de oxígeno (ROS)microscopía de iluminación estructurada (SIM)Microscopía de alta resolución

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