Video Experimental Relacionado

Updated: Jan 13, 2026

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor

Published on: October 15, 2015

Descloración de ésteres organofosforados clorados mixtos (V6 y TCEP) y expresión asociada del gen de deshalogenasa

Sen Yang^1,2,3,4, Junhong Wu^1,2,3,4, Qian Yang^1,2,3,4

¹State Key Laboratory of Advanced Environmental Technology, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou, China.

Applied and environmental microbiology

|January 6, 2026

Abstract:

Dehalococcoides mccartyi (D. mccartyi) plays a critical role in the dechlorination of halogenated organic pollutants, yet its performance on mixed chlorinated organophosphate esters (Cl-OPEs) remains poorly understood. In this study, a mixed microbial culture containing D. mccartyi was developed to dechlorinate commercial V6, a mixture comprising 80.2% 2,2-bis(chloromethyl)trimethylene bis(bis(2-chloroethyl) phosphate) (V6), 9.4% tris(2-chloroethyl) phosphate (TCEP), and other Cl-OPE impurities. Within 10 days, 99.5% of TCEP and 95.4% of V6 were dechlorinated. The presence of TCEP slightly enhanced the dechlorination of V6 compared with V6 as a purified compound. Both compounds underwent cleavage of C-O and C-Cl bonds in their chloroethoxy groups (Cl-CH₂-CH₂-O-), yielding different phosphate de-esterification products and ethene. D. mccartyi exhibited significant growth, with two reductive dehalogenase homologous (rdhA) genes co-transcribed during the dechlorination of commercial V6, purified V6, or TCEP. Metaproteomic analysis revealed that the enzymes encoded by these two genes were significantly expressed, suggesting that they may be the key enzymes mediating the dechlorination of mixed Cl-OPEs. Overall, this study provides insights into the role of D. mccartyi and its reductive dehalogenases in the natural attenuation of mixed Cl-OPEs in contaminated environments.

Importance:

Commercial V6, a chlorinated organophosphate esters mixture, is widely used in polyurethane foam and has been detected in various environmental matrices. This study is the first to elucidate the microbial transformation pathways and mechanisms of commercial V6. A mixed culture containing Dehalococcoides mccartyi was found to dechlorinate V6 and tris(2-chloroethyl) phosphate (TCEP) into phosphate de-esterification products, chloride ion, and ethene. Notably, two reductive dehalogenase genes were simultaneously transcribed and their corresponding enzymes co-expressed, indicating a key role of D. mccartyi in the natural attenuation of commercial V6 in the environment.

Palabras clave:

Dehalococcoides mccartyi TCEP ésteres organofosforados clorados V6 comercial deshalogenasa reductiva

Más Videos Relacionados

Analysis of Organochlorine Pesticides in a Soil Sample by a Modified QuEChERS Approach Using Ammonium Formate

Analysis of Organochlorine Pesticides in a Soil Sample by a Modified QuEChERS Approach Using Ammonium Formate

Published on: January 20, 2023

Isolation, Propagation, and Identification of Bacterial Species with Hydrocarbon Metabolizing Properties from Aquatic Habitats

Isolation, Propagation, and Identification of Bacterial Species with Hydrocarbon Metabolizing Properties from Aquatic Habitats

Published on: December 7, 2021

Videos de Conceptos Relacionados

Bioremediation

Bioremediation

Bioremediation is the use of prokaryotes, fungi, or plants to remove pollutants from the environment. This process has been used to remove harmful toxins in groundwater as a byproduct of agricultural run-off and also to clean up oil spills.