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Updated: Jan 13, 2026

Polymerase Chain Reaction: Basic Protocol Plus Troubleshooting and Optimization Strategies

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Published on: May 22, 2012

Un marco cinético universal para la amplificación isotérmica cuantitativa regida por la velocidad de la polimerasa,

Langjun Tang¹, Zhenyu Guo¹, Jinyong Wu¹

¹Department of Pharmaceutical & Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu 610065, China.

Nucleic acids research

|January 7, 2026

Resumen

Un nuevo modelo cinético explica la eficiencia de la amplificación isotérmica exponencial (IEA) utilizando la velocidad de la polimerasa, el tamaño del amplicón y la unión de cebadores. Este marco permite una cuantificación y optimización precisas para diversos métodos de amplificación.

Palabras clave:

amplificación isotérmica cinética modelo predictivo cuantificación viral biología molecular

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