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Análisis espacial y transcriptómico unicelular integrado revela RPL8 como biomarcador pronóstico y diana terapéutica

Jinna Tan¹, Junzhu Liang², Yaoyang Li²

¹Guangxi University of Chinese Medicine, Nanning 530200, China; First Clinical Medical College, Guangxi University of Chinese Medicine, Nanning, 530022, China.

Translational oncology

|January 7, 2026

Abstracto

Background & Aims:

Ribosomal protein L8 (RPL8) is up-regulated in hepatocellular carcinoma (HCC), yet its clinical value and microenvironment role remain unclear.

Methods:

Multi-omics (TCGA, CPTAC), scRNA-seq (GSE146115) and spatial transcriptomics were integrated; function was tested by RPL8 knockdown, proliferation/migration assays and drug-sensitivity screens.

Results:

RPL8 mRNA/protein were markedly elevated (p < 0.001) with AUC 0.95 for diagnosis. High RPL8 independently predicted shorter overall (HR 1.42) and disease-specific survival (HR 1.35). Mechanistically, RPL8 co-activated MYC/G2M signaling, rewired glutathione metabolism and correlated with cell-cycle/DNA-repair scores (p < 0.001). scRNA-seq showed selective RPL8 enrichment in malignant hepatocytes and exhausted CD8⁺T cells; spatial maps revealed tumor-confined expression inversely linked to immune infiltration (p < 0.01). Silencing RPL8 suppressed proliferation, migration and glutathione synthesis (p < 0.001), while sensitizing cells to sirolimus and sclareol (p < 0.05).

Conclusions:

RPL8 drives HCC progression and immune evasion, qualifying it as a diagnostic biomarker and therapeutic target.

Palabras clave:

Biomarcador Carcinoma hepatocelular Análisis integrado multiómico RPL8 Secuenciación unicelular Transcriptómica espacial

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